Echo 555 Acoustical Droplet Ejection System for High-throughput Screening

描述(由申请人提供)：德克萨斯大学西南高通量筛查(HTS)实验室创建并进行小分子筛查，以识别那些可以成为开发疗法的领导者或用作研究工具的分子。此外，HTS实验室还进行基因组RNAi筛选，以确定治疗药物开发的新靶点。HTS实验室管理着UTSW的化合物档案，目前包括20多万种类似药物的小有机化合物。这个复合库是用机构和NIH的资金购买的，免费提供给已经批准项目的德克萨斯大学西南分校的研究人员。HTS实验室帮助德克萨斯大学西南研究人员开发能够以HTS格式进行筛选的化验方法，无论是由HTS实验室在当地进行筛选，还是作为MLPCN的一部分进行筛选。HTS实验室对筛选数据进行筛选、分析、收集和分析。HTS实验室进行二次筛选，以验证在初步筛选实验中确定的感兴趣的化合物。HTS实验室的科学家与NCI资助的计划项目的研究人员、西蒙斯综合癌症中心的成员或其他NIH资助的研究人员合作，对HTS实验中确定的感兴趣的化合物进行优先排序，并管理这些化合物的再供应和存储。HTS核心维护一个筛选数据的Oracle数据库，以便可以将任何HTS实验中感兴趣的化合物的活性与使用UTSW化合物文件进行的所有其他HTS实验进行比较。除了进行HTS筛选外，HTS实验室还为德克萨斯大学西南分校的学生、博士后研究员、科研人员和教职员工提供HTS实验开发和执行方面的培训和建议。

DESCRIPTION (provided by applicant): The UT Southwestern high-throughput screening (HTS) laboratory creates and conducts screens of small molecules to identify those that can become leads for developing therapeutics or used as tools in research. In addition, the HTS laboratory conducts genomic RNAi screens to identify novel targets for the development of therapeutics. The HTS laboratory manages the UTSW chemical compound file currently including more than 200,000 drug-like small organic compounds. This compound library, which was purchased with institutional and NIH funds, is provided at no cost to UT Southwestern Investigators who have approved projects. The HTS laboratory aids UT Southwestern investigators in developing assays that are capable of being screened in HTS format, either locally by the HTS laboratory or as part of the MLPCN. The HTS laboratory screens assays and collects and analyzes screening data. The HTS lab conducts secondary screens required to validate compounds of interest identified during primary screening experiments. Working with the investigators of a NCI-funded Program Project, members of the Simmons Comprehensive Cancer Center, or other NIH-funded investigators, scientists in the HTS laboratory prioritize the compounds of interest identified in HTS experiments and manage resupply and storage of those compounds. The HTS core maintains an Oracle database of screening data so that the activity of compounds of interest in any HTS experiment can be compared to all other HTS experiments conducted with the UTSW compound file. In addition to conducting HTS screens, the HTS laboratory trains and advises students, postdoctoral fellows, staff scientists and faculty at UT Southwestern on the development and execution of HTS experiments.