Immunology Core

免疫学核心

• 批准号: 7226416
• 负责人: REBECCA D ADKINS
• 金额: $ 5.16万
• 依托单位: UNIVERSITY OF MIAMI SCHOOL OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-12-01 至 2012-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7226416
• 关键词: Animals; Antigens; Cell Separation; Cells; Data; Development; Evaluation; Frequencies; Granzyme; Immune; Immunology; Individual; Measurement; Measures; Preparation; Protocols documentation; Regulation; Research Personnel; Services; Sorting - Cell Movement; Tumor Immunity; Work; cell preparation; cytokine; design; enzyme linked immunospot assay; magnetic beads; programs; protocol development

The Immunology Core will serve as a critical component of the "Regulation of Anti-Tumor Immunity" Program, providing services and expertise in both cell isolation and purification and the measurement of antigen specific effector functions. The Core will provide preparative cell isolation using magnetic bead sorting with the autoMACS Separator. The Core will also provide analyses of the frequencies of cells expressing cytokines and other effector molecules (e.g., granzyme) by ELISPOT using the C.T.L. ImmunoSpot automated ELISPOT counter. Lastly, the Core will work with individual investigators on the technical design of cell preparation and animal manipulation and in the interpretation of experimental data.

免疫学核心将作为“抗肿瘤免疫调节”的关键组成部分 计划，提供细胞分离和纯化以及细胞测量方面的服务和专业知识 抗原特异性效应子功能。 Core将使用autoMACS分离器通过磁珠分选提供制备型细胞分离。 核心还将提供表达细胞因子和其他效应因子的细胞频率的分析。 分子（例如，颗粒酶）通过ELISPOT使用C.T.L. ImmunoSpot自动ELISPOT计数器。最后， 核心小组将与个别研究人员合作，进行细胞制备和动物实验的技术设计， 操作和实验数据的解释。