THE EFFECT OF BEEF AND HEME IRON ON ZINC ABSORPTION IN CHILDREN

该子项目是利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得主要资金， 因此可以在其他CRISP条目中表示。列出的机构是 中心，不一定是研究者的机构。 研究提案的目的：（1）量化血红素铁对锌吸收的影响，并证明血红素铁的吸收是否受锌摄入量的影响。（2）评估食用牛肉蛋白对膳食中非血红素铁和无机锌吸收的影响。 研究项目描述：将开展两项研究方案。48名4 - 8岁的儿童将被随机分配接受含有三种剂量的内在标记血红素铁和两种剂量的锌的膳食。将使用已建立的稳定同位素方法测量血红素铁和锌的吸收，以检验血红素铁不干扰锌吸收以及锌不干扰血红素铁吸收的假设。在第二个方案中，32名4-8岁的儿童将随机接受基于牛肉蛋白或大豆蛋白的膳食。随餐提供的面包要用铁和锌的稳定同位素标记。铁和锌的吸收将使用已建立的稳定同位素方法进行测量，以检验与大豆蛋白相比，牛肉蛋白增强饮食中非血红素铁和无机锌吸收的假设。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Objectives of Research proposal: (1) To quantify the effect of heme iron on zinc absorption, and to demonstrate whether heme iron absorption is affected by zinc intake, (2) To assess the effect of consumption of beef protein on non-heme iron and inorganic zinc absorption from the diet. Description of Research Project: Two protocols will be carried out. Forty-eight children aged between 4 and 8 years will be randomized to receive a meal containing one of three doses of intrinsically labeled heme iron and one of two doses of zinc. Absorption of the heme iron and the zinc will be measured using established stable isotope methods, to test the hypotheses that heme iron does not interfere with zinc absorption, and that zinc does not interfere with heme iron absorption. In a second protocol, 32 children aged 4-8 years will be randomized to receive a meal based on beef protein or soy protein. Bread given with the meal with be labeled with iron and zinc stable isotopes. Iron and zinc absorption will be measured using established stable isotope methods to test the hypothesis that beef protein enhances non-heme iron and inorganic zinc absorption from the diet, when compared to soy protein.