HORMONAL REGULATION OF GENE EXPRESSION DURING LACTATION IN HUMANS
人类哺乳期间基因表达的激素调节
基本信息
- 批准号:7605906
- 负责人:
- 金额:$ 0.41万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2007
- 资助国家:美国
- 起止时间:2007-02-15 至 2007-11-30
- 项目状态:已结题
- 来源:
- 关键词:AcuteAmino AcidsBreastBreast FeedingComputer Retrieval of Information on Scientific Projects DatabaseDoseFailureFastingFoodFundingGalactoseGene ExpressionGlucoseGrantHormonalHormonesHumanHuman MilkInstitutionInvestigationLactalbuminLactationLactoseMammary glandMessenger RNAMilkMilk ProteinsMothersNumbersPhosphoenolpyruvate CarboxylasePlasmaPremature InfantProductionProtein BiosynthesisRateResearchResearch PersonnelResourcesRiskSLC2A1 geneSomatropinSourceSyndromeTestingTissuesUnited States National Institutes of HealthWestern EuropeWomanabstractingalpha Lactalbumindayexperiencefeedinghepatic gluconeogenesishormone regulationinterestmRNA Expressionmilk fat globuler-hGH-Mteenage mothertreatment duration
项目摘要
This subproject is one of many research subprojects utilizing the
resources provided by a Center grant funded by NIH/NCRR. The subproject and
investigator (PI) may have received primary funding from another NIH source,
and thus could be represented in other CRISP entries. The institution listed is
for the Center, which is not necessarily the institution for the investigator.
ABSTRACT
Over the last two decades, renewed interest and expertise in and about breastfeeding has occurred in the U.S. and Western Europe. Human milk is universally accepted as the ideal food for infnants and breastfeeding has real and potential benefits for the mother. A number of these women have been unsuccessful in their attempts to breastfeed (insufficient milk syndrome) and others are known to be at high risk of breastfeeding failure (women with premature infants and teenage mothers). The primary determinant of milk volume is lactose production. We have previously demonstrated that following a 24 hr fast, lactating women have a 35% higher rate of glucose turnover than controls, tolerate a 24 hr fast without difficulty and have no differences in substrate or hormone concentrations when compared to controls. In addition, we demonstrated: 1. the human breast was capable of de novo synthesis of both the glucose and galactose moieties of lactose (hexoneogenesis), 2. The fraction of lactose derived from hexoneogenesis increased from 30% to nearly 50% comparing the fed to the 24 hr fasted values, 3. a source of amino acids other than the plasma free amino acid pool contributes to milk protein synthesis, and 4. human growth hormone (rhGH) increases human milk production and hepatic gluconeogenesis. Finally we demonstrated that milk fat globules contain sufficient specifc mRNA to permit investigations of the substrate and hormonal factors that regulate gene expression in the lactating breast. Understanding whether and how the mRNA in the milk fat globules are regulated is the initial focus of these studies. Because of our previous experience with rhGH, we will initially test the acute effect of rhGH on alpha lactalbumen mRNA expression in milk fat globules.
HYPOTHESIS
The Hypothesis to be tested is that rhGH will increase the expression of the gene for alpha-lactalbumin and GLUT 1 and 3 but not PEPCK following 4 days of treatment at a dose of 0.1 mg/kg-day.
SPECIFIC AIMS
To determine whether rhGH acutely increases the expression of a-lactalbumin in human mammary tissue.
这个子项目是许多研究子项目中利用
资源由NIH/NCRR资助的中心拨款提供。子项目和
调查员(PI)可能从NIH的另一个来源获得了主要资金,
并因此可以在其他清晰的条目中表示。列出的机构是
该中心不一定是调查人员的机构。
摘要
在过去的二十年里,美国和西欧重新燃起了人们对母乳喂养的兴趣和专业知识。母乳被普遍认为是婴儿的理想食物,母乳喂养对母亲有实际和潜在的好处。其中一些妇女的母乳喂养尝试不成功(乳汁不足综合症),其他妇女(早产儿和十几岁母亲的妇女)母乳喂养失败的风险很高。乳糖量的主要决定因素是乳糖产量。我们之前已经证明,在禁食24小时后,哺乳期妇女的葡萄糖周转率比对照组高35%,耐受24小时禁食没有困难,底物或激素浓度与对照组相比没有差异。此外,我们还证明:1.人的乳房能够从头合成葡萄糖和半乳糖部分的乳糖(己酮合成);2.与24小时禁食相比,来自6酮合成的乳糖比例从30%增加到近50%;3.血浆游离氨基酸库以外的氨基酸来源对乳蛋白合成有贡献;4.人生长激素(RhGH)增加了人的乳产量和肝脏的糖异生。最后,我们证明了乳脂小球含有足够的特异性信使核糖核酸,使得研究调节乳房基因表达的底物和激素因素成为可能。了解乳脂球中的信使核糖核酸是否以及如何受到调控是这些研究的最初重点。由于我们之前使用重组人生长激素的经验,我们将首先测试重组人生长激素对乳脂球中α-乳蛋白mRNA表达的急性影响。
假设
需要检验的假设是,重组人生长激素在0.1 mg/kg·d的剂量下治疗4天后,将增加α-乳蛋白和GLUT 1和3的基因表达,但不增加PEPCK的表达。
具体目标
目的:研究重组人生长激素是否能显著增加人乳腺组织中α-乳清蛋白的表达。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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MOREY W HAYMOND的其他文献
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Glucagon Mini-Dosing Pen for Treatment of Hypoglycemia
用于治疗低血糖的胰高血糖素小剂量笔
- 批准号:
8781802 - 财政年份:2013
- 资助金额:
$ 0.41万 - 项目类别:
Glucagon Mini-Dosing Pen for Treatment of Hypoglycemia
用于治疗低血糖的胰高血糖素小剂量笔
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8521920 - 财政年份:2013
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$ 0.41万 - 项目类别:
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儿童营养研究中心培训计划
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8547086 - 财政年份:2012
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$ 0.41万 - 项目类别:
The Childrens Nutrition Research Center Training Program
儿童营养研究中心培训计划
- 批准号:
8267142 - 财政年份:2012
- 资助金额:
$ 0.41万 - 项目类别:
The Childrens Nutrition Research Center Training Program
儿童营养研究中心培训计划
- 批准号:
8658130 - 财政年份:2012
- 资助金额:
$ 0.41万 - 项目类别:
THE ROLE OF DIETARY CARBOHYDRATE ON MATERNAL FAT MOBILIZATION AND OXIDATION D
膳食碳水化合物对母体脂肪动员和氧化的作用 D
- 批准号:
8166688 - 财政年份:2009
- 资助金额:
$ 0.41万 - 项目类别:
A NOVEL THERAPEUTIC MODALITY FOR CONGENITAL ADRENAL HYPERPLASIA
先天性肾上腺增生症的新治疗方式
- 批准号:
8166690 - 财政年份:2009
- 资助金额:
$ 0.41万 - 项目类别:
A NOVEL THERAPEUTIC MODALITY FOR CONGENITAL ADRENAL HYPERPLASIA
先天性肾上腺增生症的新治疗方式
- 批准号:
7950638 - 财政年份:2008
- 资助金额:
$ 0.41万 - 项目类别:
THE ROLE OF DIETARY CARBOHYDRATE ON MATERNAL FAT MOBILIZATION AND OXIDATION D
膳食碳水化合物对母体脂肪动员和氧化的作用 D
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7950636 - 财政年份:2008
- 资助金额:
$ 0.41万 - 项目类别:
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不同吸奶方案对 α 乳清蛋白 mRNA 浓度的影响
- 批准号:
7605888 - 财政年份:2007
- 资助金额:
$ 0.41万 - 项目类别:
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