EXERCISE, NUTRITION, AND SARCOPENIA IN OLDER ADULTS

老年人的运动、营养和肌肉减少症

• 批准号: 7605424
• 负责人: HANS DRYER
• 金额: $ 2.83万
• 依托单位: UNIVERSITY OF TEXAS MED BR GALVESTON
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-04-01 至 2008-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7605424
• 关键词: Aerobic; Amino Acids; Bacteria; Biological Markers; Biopsy; Blood; Blood Pressure; Blood Tests; Catheters; Central City; Class; Coagulation Process; Computer Retrieval of Information on Scientific Projects Database; Count; DEXA; Daily; Dermabond; Dietitian; Eating; Elderly; Electrolytes; Exercise; Experimental Designs; Fever; Food; Funding; Glues; Grant; Growth; Height; Hour; Human; Individual; Infection; Infusion procedures; Institution; Instruction; Intake; Intervention; Isotopes; Leg; Local Anesthetics; Measures; Medicine; Muscle; Numbers; Nurses; Nutritional; Pain; Procedures; Proteins; Protocols documentation; Radioactive; Research; Research Personnel; Resistance; Resources; Salts; Scanning; Schedule; Skin; Skin Tissue; Source; Sterility; Surgical incisions; Test Result; Testing; Training; Training Programs; United States National Institutes of Health; Upper arm; Water; Week; Weight; Weight Lifting; aging population; base; day; dietary supplements; drinking; improved; instrument; liver function; microorganism; nutrition; response; sarcopenia; stable isotope; sugar; volunteer

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Background: Nutritional intake and weight lifting exercise can both stimulate muscle growth. However, nutritional intake (such as regular meal) does not promote as much muscle growth in senior citizens as compared to younger individuals. Hypothesis: We hypothesize that an aquatic exercise training (combining both aerobic and weight lifting exercise) will maximize the muscle growth when combined with regular meal intake. Specific Aims and Procedures (summary): 1) To determine which molecular markers involved in muscle growth are stimulated with nutritional intake in young and older volunteers. 2) To determine if a 12-week aquatic exercise training program (utilizing both aerobic and weight lifting exercises) will improve the muscle growth in response to a nutritional intake. Experimental Design (summary): Volulnteers will come to the GCRC the afternoon before the study. The nurses will measure their weight, height, blood pressure, and the nurses will draw blood to check again blood count, clotting, and some other blood tests including electrolytes (salts) and liver function. Should these tests result abnormal, volunteers will not be allowed to participate to the study. A DEXA scan will also be performed. Stable isotopes. After the catheters are placed, volunteers will be given stable isotopes (infusion) through one of the arm catheters. This infusion will go on until the end of the study, about 7 hours. In general, stable isotopes are of a material very similar to the regular material our body is made of, except that they are heavier (but not radioactive). Stable isotopes are natural compounds also present in very small quantities in your body. The isotopes are tested in order to assure their sterility (absence of microorganisms, such as bacteria, that may give infections and fever). We will then be able, using highly sophisticated analytical instruments, to measure the small changes in the concentration of these substances in your blood, breath, and muscle. Muscle biopsies. During the study we will take 4 muscles biopsies from the same leg in which we placed the catheters. This procedure involves the taking of a small piece of muscle from your leg. The skin is cleaned and made sterile, and the skin and tissue below are injected with local anesthetic (numbing medicine) to eliminate pain After the last biopsy the incision will be closed with one stitch or a special skin glue (Dermabond). Blood draws. The total amount of blood drawn during the study will be approximately 150 ml (approximately 5 ounces or 10 tablespoons). Nutritional Supplement. During the study you will drink a nutritional supplement that contains sugar and protein (amino acids) one hour prior to exercising or one hour after completing the weight lifting exercise. (3) Aquatic Exercise Training (senior volunteers) 24-Hour food record. You will be asked to record your daily food intake in the form provided by us for three days during the same week (two week days and one weekend day) at the start of the training (1st week), during 4th, 8th, and last (12th) week of training. Dr. Inniss (research dietitian) will give you an instruction how to fill out the form. Aquatic Exercise Training. You will perform aquatic exercise (combination of aerobic and resistance exercise in the water) at the TideWay Aquatic Therapy Center (6444 Central City Blvd, Galveston TX 77551) instructed by certified trainers. You will be asked to attend three scheduled classes each week for 12 weeks consecutively. Each exercise session will be performed entirely in the water which lasts approximately for 45 minutes. (4) Infusion Study (Post-training) (senior volunteers) At least 48 hours after the last exercise session, you will be admitted at GCRC and whole body DEXA scan will be performed. After an overnight fast, you will perform another infusion study as described above in (2) Infusion Protocol. Significance (summary): These studies will provide regarding the mechanisms that regulate human muscle growth, and will be utilized as a basis from which to develop interventions for improving muscle growth in aging population.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 背景：营养摄入和举重运动都能刺激肌肉生长。 然而，与年轻人相比，营养摄入（如正常膳食）并不能促进老年人的肌肉生长。 假设：我们假设，水上运动训练（结合有氧运动和举重运动）将最大限度地提高肌肉生长时，结合定期进食。 具体目标和程序（摘要）： 1)确定哪些参与肌肉生长的分子标志物在年轻和老年志愿者中受到营养摄入的刺激。 2)确定12周的水上运动训练计划（利用有氧运动和举重运动）是否会改善肌肉生长对营养摄入的反应。 实验设计（总结）：志愿者将在研究前一天下午来到GCRC。护士将测量他们的体重，身高，血压，护士将抽血再次检查血细胞计数，凝血和其他一些血液检查，包括电解质（盐）和肝功能。如果这些检查结果异常，则不允许志愿者参与研究。 还将进行DEXA扫描。 稳定同位素。放置导管后，将通过其中一根手臂导管向志愿者提供稳定同位素（输注）。该输注将持续至研究结束，约7小时。一般来说，稳定同位素的物质与构成我们身体的常规物质非常相似，只是它们更重（但不具有放射性）。 稳定同位素是一种天然化合物，在你的身体中也有很小的数量。对同位素进行测试是为了确保它们的无菌性（没有微生物，如细菌，可能会引起感染和发烧）。然后，我们将能够使用高度复杂的分析仪器，测量这些物质在您的血液，呼吸和肌肉中浓度的微小变化。 肌肉活检。在研究期间，我们将从放置导管的同一条腿上采集4份肌肉活检。这个过程包括从你的腿上取下一小块肌肉。 皮肤被清洁和消毒，皮肤和组织下面注射局部麻醉剂（麻木药物），以消除疼痛后，最后一次活检的切口将关闭一针或特殊的皮肤胶（Dermabond）。 抽血。研究期间抽取的血液总量约为150 ml（约5盎司或10汤匙）。 营养补充剂。 研究期间，您将在运动前1小时或完成举重运动后1小时饮用含有糖和蛋白质（氨基酸）的营养补充剂。 (3)水上运动训练（高级志愿者） 24-小时食物记录。 您将被要求在培训开始（第1周）、培训第4周、第8周和最后一周（第12周）的同一周（两个工作日和一个周末）内，在我们提供的表格中记录您的每日食物摄入量。 Inniss博士（研究营养师）将指导您如何填写表格。 水上运动训练。 您将在TideWay Aquatic Therapy Center（6444中心城市Blvd，加尔维斯顿TX 77551）进行水上运动（水中有氧运动和阻力运动的组合），由认证教练指导。 你将被要求参加每周三个预定的课程，连续12周。 每次运动都将完全在水中进行，持续约45分钟。 (4)输注研究（培训后）（老年志愿者） 在最后一次运动后至少48小时，您将在GCRC住院，并进行全身DEXA扫描。 禁食过夜后，您将按照上文（2）输注方案中所述进行另一项输注研究。 意义（总结）：这些研究将提供关于调节人类肌肉生长的机制，并将被用作制定干预措施以改善老龄人口肌肉生长的基础。