STUDIES OF HUMAN TOOTH INTEGUMENTS

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。列出的机构是 研究中心，而研究中心不一定是研究者所在的机构。 本临床研究计划的目的是研究在生物膜形成的早期非生长阶段，细菌定植者在天然牙齿表面的体内粘附。 我们将使用棋盘DNA-DNA杂交试验来表征微生物物种的早期附着。 将从健康志愿者和牙龈炎患者中取样体内薄膜。 本研究将评估血清唾液混合物涂布牙面的附着效果，并评估不同牙列环境下形成的薄膜的附着效果。 目的是进一步了解口腔环境的矿物质-蛋白质界面，并建立牙周病原体和其他有害微生物物种在表面定植的参数。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The goal of this clinical research proposal is to investigate in vivo adherence of bacterial colonizers on natural tooth surfaces in the early non-growth phase of biofilm formation. We will use the checkerboard DNA-DNA hybridization assay to characterize the early attachment of microbial species. In vivo pellicle will be sampled from healthy volunteers and gingivitis patients. We will evaluate the attachment effect of tooth surfaces coated with serum saliva mixtures and evaluate the attachment effect of pellicle formed in different environments of dentition. The goal is to further understand the mineral-protein interface of oral environment and establish parameters involved in colonization of the surface by periodontal pathogens and other less deleterious microbial species.