STUDIES OF HUMAN TOOTH INTEGUMENTS

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。这项临床研究计划的目标是研究细菌定殖体在生物膜形成的早期非生长阶段在天然牙齿表面的体内黏附。我们将使用棋盘DNA-DNA杂交试验来表征微生物物种的早期附着。活体膜将从健康志愿者和牙周炎患者身上采集。我们将评估涂有血清唾液混合物的牙齿表面的附着效果，以及在不同的牙列环境中形成的膜的附着效果。目的是进一步了解口腔环境中的矿物-蛋白质界面，并建立与牙周病原体和其他危害较小的微生物物种在表面定植有关的参数。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The goal of this clinical research proposal is to investigate in vivo adherence of bacterial colonizers on natural tooth surfaces in the early non-growth phase of biofilm formation. We will use the checkerboard DNA-DNA hybridization assay to characterize the early attachment of microbial species. In vivo pellicle will be sampled from healthy volunteers and gingivitis patients. We will evaluate the attachment effect of tooth surfaces coated with serum saliva mixtures and evaluate the attachment effect of pellicle formed in different environments of dentition. The goal is to further understand the mineral-protein interface of oral environment and establish parameters involved in colonization of the surface by periodontal pathogens and other less deleterious microbial species.