STUDIES OF HUMAN TOOTH INTEGUMENTS

人类牙齿外皮的研究

• 批准号: 7379537
• 负责人: Frank G Oppenheim
• 金额: $ 11.2万
• 依托单位: BOSTON UNIVERSITY MEDICAL CAMPUS
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-12-01 至 2006-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7379537
• 关键词: STUDIES; HUMAN; TOOTH; INTEGUMENTS

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The goal of this clinical research proposal is to investigate in vivo adherence of bacterial colonizers on natural tooth surfaces in the early non-growth phase of biofilm formation. We will use the checkerboard DNA-DNA hybridization assay to characterize the early attachment of microbial species. In vivo pellicle will be sampled from healthy volunteers and gingivitis patients. We will evaluate the attachment effect of tooth surfaces coated with serum saliva mixtures and evaluate the attachment effect of pellicle formed in different environments of dentition. The goal is to further understand the mineral-protein interface of oral environment and establish parameters involved in colonization of the surface by periodontal pathogens and other less deleterious microbial species.

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子弹和调查员（PI）可能已经从其他NIH来源获得了主要资金，因此可以在其他清晰的条目中代表。列出的机构适用于该中心，这不一定是调查员的机构。这项临床研究建议的目的是研究生物膜形成早期的非生长阶段的天然牙齿表面上细菌殖民者的体内粘附。我们将使用棋盘DNA-DNA杂交测定法来表征微生物物种的早期附着。体内颗粒将从健康的志愿者和牙龈炎患者中取样。我们将评估涂有血清唾液混合物的牙齿表面的固定效果，并评估在不同牙齿环境中形成的细胞的附着效应。目的是进一步了解口服环境的矿物质蛋白界面，并通过牙周病原体和其他不太有害的微生物物种建立参数参与表面定植的参数。