SALINE/DIURETIC STUDY

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 我们建议进行一项研究，目的是为钠滞留的遗传学研究产生表型，钠滞留是一种易患高血压的疾病。我们集中在三个肾单位区域，即粗大的升肢，其中钠，钾，2氯协同转运体运作，在远曲小管中的氯化钠协同转运体，以及在集合管中的上皮性钠通道。两百名无关的受试者，黑人和白人人数相等，男性和女性，年龄在18岁到35岁之间，都将参加。受试者将在研究前一天下午被送往综合临床研究中心，在那里他们将接受高钠饮食。第二天早上，一升生理盐水将在3个小时内注入。我们将评估受试者如何排泄(或保持)钠负荷(观察将在4小时内进行)。然后，受试者将被给予一种“循环利尿剂”，速尿，在30分钟内静脉注射10毫克，然后观察4个小时。父母中的一方或双方将提供DNA血样，以便进行额外的遗传不平衡遗传测试。这将是开发钠滞留表型和血压对钠敏感性的重要方案。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We propose a study for the purpose of generating phenotypes for genetic studies of sodium retention, a condition that predisposes to high blood pressure. We focus on three nephron regions, the thick ascending limb where the sodium, potassium, 2 chloride co-transporter operates, the sodium-chloride co-transporter in distal convoluted tubule, and the epithelial sodium channel in the collecting duct. Two hundred unrelated subjects, equal numbers of blacks and whites, males and females, ages 18-35 years, will participate. Subjects will be admitted to the General Clinical Research Center the afternoon before study where they will receive a diet high in sodium. The following morning, one liter of normal saline will be infused over 3 hours. We will assess how subjects excrete (or hold onto) the sodium load (observations will be made over 4 hours). Then subjects will be given a "loop-diuretic," furosemide, 10 mg intravenously over 30 minutes, and then observed over a period of 4 hours. One or both parents will give a blood sample for DNA to allow for performing the additional genetic testing of transmission disequilibrium. THis will be an important protocol for developing the phenotypes of sodium retention, and sensitivity of the blood pressure to sodium.