THE EPIDEMIOLOGY OF HOME ALLERGENS AND ASTHMA

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 对遗传上易患哮喘的出生队列进行前瞻性纵向研究是确定暴露、血清学反应、细胞反应和疾病结局之间的免疫学关系的最合适的研究设计。我们建议利用一项针对过敏或哮喘父母的儿童进行的前瞻性纵向队列研究，以检查婴儿接触变应原在以下发展中的作用：(1)5岁时对这些过敏原的抗体和细胞免疫反应；(2)皮肤致敏、支气管反应和7岁时的哮喘/喘息。队列中505名儿童的过敏原暴露包括屋尘螨(Der p 1，Der f 1)、真菌、蟑螂(Bla g 1，B la g 2)、猫、(Fel D I)和狗(Can I)。抗体结果包括总IgE和过敏原特异性IgE、Ig G、Ig G1和Ig G4。在父母患有哮喘的50名5岁儿童中，25名为高抗原暴露组，25名为低抗原暴露组，我们将以淋巴细胞增殖和细胞因子产生(IL-4、IL-5和干扰素)的形式来分析PHA、螨虫、猫和蟑螂刺激下的后续反应。(干扰素？))。其他要检查的潜在影响宿主和环境因素包括：父母哮喘史、母亲产前抗体状况、围产期病史、急性下呼吸道病史、日托、环境烟草烟雾、性别和种族。 我们假设，到5岁时，以下因素将分别有助于预测儿童的总IgE和过敏原特异性抗体反应： 父母有哮喘史和产前母体抗体水平。 2岁以前的下呼吸道疾病史、环境烟草烟雾暴露、性别和种族。 C.在生命的第一年，过敏原在家中的分布和暴露水平。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. A longitudinal prospective study of a birth cohort genetically predisposed to developing asthma is the most appropriate study design to determine the immunologic relationship between exposure, serologic response, cellular response, and disease outcome. We propose to utilize a prospective longitudinal cohort study of children of allergic or asthmatic parents to examine the role of infant allergen exposure in the development of (1) antibody and cellular immune responses to these allergens by age 5; (2) skin sensitization, bronchial responsiveness, and asthma/wheeze by age 7. The allergen exposures for the 505 children in the cohort include house dust mite (Der p 1, Der f 1), fungi, cockroach (Bla g 1, Bla g 2), cat, (Fel d I), and dog (Can I). Antibody outcomes include total IgE and allergen specific IgE, IgG, IgG1 and IgG4. In a subset of 50 five year old children with asthmatic parents, 25 with high and 25 with low antigen exposure in infancy, we will assay subsequent response to PHA, mite, cat and cockroach stimulation in the form of lymphocyte proliferation and cytokine production (IL-4, IL-5, and interferon-? (IFN-?)). Additional potentially influential host and environmental factors to be examined include: parent asthma history, maternal prenatal antibody status, perinatal history, acute lower respiratory illness history, day care, environmental tobacco smoke, sex, and race. We hypothesize that by 5 years of age, the following factors will each contribute to the prediction of the total IgE and allergen specific antibody response of the child: a. Parental history of asthma and prenatal maternal antibody levels. b. Lower respiratory illness history before age 2, environmental tobacco smoke exposure, sex and race. c. Allergen distribution and exposure levels in the home during the first year of life.