IDENTIFICATION OF PROTEIN INTERACTIONS WITH TURTLE TOLLOID-LIKE PROTEIN

鉴定蛋白质与海龟类蛋白的相互作用

• 批准号: 7627583
• 负责人: BORIS SABIRZHANOV
• 金额: $ 0.65万
• 依托单位: UNIVERSITY OF SOUTH DAKOTA
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-06-01 至 2008-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7627583
• 关键词: Biological Assay; Brain Stem; Complement Factor B; Computer Retrieval of Information on Scientific Projects Database; Data; Embryo; Embryonic Development; Family; Family member; Funding; Genes; Grant; In Vitro; Institution; Metalloproteases; Morphogenesis; Pattern; Play; Preparation; Proteins; Recombinants; Regulation; Research; Research Personnel; Resources; Role; Source; Synaptic plasticity; Tissues; Turtles; United States National Institutes of Health; conditioning; neurogenesis; neurotrophic factor; receptor; trafficking

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. It is clear that the tolloid family of metalloproteases play an important role in embryonic patterning and tissue morphogenesis during embryogenesis. Evidence suggests that the tolloids facilitate the activity of neurotrophic factors such as the BMPs and may function in regulation of neurogenesis and mechanisms of synaptic plasticity. Our preliminary data show that expression of turtle tolloid-like gene (tT11) is significantly upregulated during conditioning compared to pseudoconditioned controls in vitro brain stem preparations. What are the mechanisms of action of tolloid-like genes, specifically tTLL. We hypothesize that tTLL can potentiate the activity of transforming frowth factor-B (TGF-B) family member, by antagonizing Chrodih, and/or tTLL can act by different mechanisms (tTLL can be involved in receptor trafficking or it can interact with some extacellular matrix component). To understand this question we will try to get two recombinant tTLL proteins (active and inactive) and perform in vitro cleavage assay of these two tTLLs with Chordih. These two aims will help us to achiee our next aim: to identify protein interaction with turtle tolloid-like protein.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 很明显，tolloid家族的金属蛋白酶在胚胎发育过程中的胚胎模式和组织形态发生中起着重要作用。有证据表明，tolloids促进神经营养因子如BMP的活性，并可能在神经发生和突触可塑性机制的调节中起作用。我们的初步数据表明，海龟tolloid样基因（tT 11）的表达显着上调，在空调相比，假条件控制在体外脑干制备。tolloid样基因的作用机制是什么，特别是tTLL。我们假设tTLL可以通过拮抗Chrodih增强转化生长因子-B（TGF-B）家族成员的活性，和/或tTLL可以通过不同的机制起作用（tTLL可以参与受体运输或它可以与一些细胞外基质组分相互作用）。为了理解这个问题，我们将尝试获得两种重组tTLL蛋白（活性和非活性），并用Chordih进行这两种tTLL的体外切割测定。这两个目标将帮助我们实现我们的下一个目标：确定蛋白质与海龟tolloid样蛋白的相互作用。