A NEW MODEL FOR FEAR LEARNING IN TROUT

鳟鱼恐惧学习的新模型

• 批准号: 7627585
• 负责人: CLIFF H SUMMERS
• 金额: $ 0.48万
• 依托单位: UNIVERSITY OF SOUTH DAKOTA
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-06-01 至 2008-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7627585
• 关键词: Adopted; Aggressive behavior; Auditory; Behavioral; Brain; Brain region; Computer Retrieval of Information on Scientific Projects Database; Condition; Conditioned Stimulus; Elements; Environment; Fishes; Fright; Funding; Grant; Hydrocortisone; Institution; Learning; Lizards; Microdissection; Modeling; Neuropeptides; Oncorhynchus mykiss; Plasma; Polymerase Chain Reaction; Receptor Gene; Research; Research Personnel; Resources; Rodent Model; Role; Route; Salmo trutta; Source; Stimulus; Stress and Coping; Testing; Time; United States National Institutes of Health; Work; conditioned fear; day; design; morris water maze; response; size; social; social stress; somatosensory; stressor

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We propose to develop a new model for fear conditioning using rainbow trout. This model combines elements discovered during our recent work on social stress in lizards and rainbow trout with well defined rodent models for spatial learning (Morris water maze) and fear conditioning. Social aggression is the most potent stressor known, which we utilize in the form of a march larger intruder fish as an unconditioned stimulus (US). This social aggression US is paired with an auditory/somatosensory conditioned stimulus (CS) in context of an escape route (hole) available only to the smaller resident test fish (due to size). Fish either learn to escape from social aggression or remain in the tank to be attacked and subordinated. After seven days of CS-US paairing, on the eighth day the CS is presented alone (without the larger fish), and fish respond either by escaping or by a physiologically conditioned response, elevated plasma cortisol. The model is designed to be used for testing spatial learning in a highly fearful situation, and producing fear conditioning using contextually and ecologically relevant stimuli and behavioral responses. We hypothesize that the neuropeptide CRF regulates which stress coping strategies are adopted by trout during learning in a socially aggressive environment. The aims of this proposal are to: 1. Test and refine the experimental parameters of the model 2. Prove that we can obtain repeatable results 3. Begin to distinguish the role of the CRF in stress coping strategies during fear learning 4. Compare expression of CRF and CRF receptor genes in specific brain regions of trout that do and don't learn to escape using brain punch microdissection and real-time PCR

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 我们建议使用虹鳟鱼开发一种新的恐惧条件反射模型。这个模型结合了我们最近在蜥蜴和虹鳟鱼的社会压力研究中发现的元素，以及定义良好的啮齿动物空间学习模型（莫里斯水迷宫）和恐惧条件反射。社会攻击是已知的最强有力的应激源，我们以一种更大的入侵鱼类作为无条件刺激的形式利用它。这种社会侵略美国配对听觉/体感条件刺激（CS）的逃生路线（孔）的上下文中，只有较小的居民测试鱼（由于大小）。鱼要么学会逃避社会的侵略，要么留在坦克被攻击和服从。 CS-US配对7天后，在第8天，CS单独出现（没有较大的鱼），鱼的反应是逃避或生理条件反应，血浆皮质醇升高。该模型被设计用于测试空间学习在一个高度恐惧的情况下，并产生恐惧条件反射使用上下文和生态相关的刺激和行为反应。我们假设，神经肽CRF调节鳟鱼在学习过程中采取的应对压力的策略，在社会侵略性的环境。这项建议的目的是： 1.对模型的实验参数进行测试和修正 2.证明我们可以获得可重复的结果 3.开始区分CRF在恐惧学习期间压力应对策略中的作用 4.使用脑穿孔显微切割和实时PCR比较鳟鱼学习和不学习逃跑的特定脑区域中CRF和CRF受体基因的表达