LACRIMAL GLAND BIOINFORMATICS: A NEURAL CONNECTION OF DRY EYE AND AGING

泪腺生物信息学：干眼症与衰老的神经联系

• 批准号: 7609948
• 负责人: DOAN M NGUYEN
• 金额: $ 18万
• 依托单位: LOUISIANA STATE UNIV A&M COL BATON ROUGE
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-05-01 至 2008-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7609948
• 关键词: Affect; Age; Aging; Autonomic Pathways; Bioinformatics; Biological Response Modifiers; Cellular Structures; Computer Retrieval of Information on Scientific Projects Database; Cornea; Corneal Injury; Data; Denervation; Endoplasmic Reticulum; Esthesia; Excision; Eye diseases; Funding; Gene Expression; Gene Expression Profiling; Genes; Grant; Inflammation; Institution; Internet; Lacrimal gland structure; Liquid substance; Maintenance; Molecular Profiling; Optics; Pattern; Peripheral; Proteins; Research; Research Personnel; Resources; Sensory; Services; Source; Surface; Testing; Tissues; Transcriptional Regulation; United States National Institutes of Health; Vision; eye dryness; nerve supply; neural circuit; neuroregulation; ocular surface; pathogen; relating to nervous system; response

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The lacrimal gland tissue is of critical importance to good vision. It is the major contributor of proteins and fluid in tears that help to maintain the optical surface of the cornea, to protect the cornea from pathogens, and to maintain the cellular structure of the ocular surface. Activation of lacrimal gland secretion is under neural control. Sensory (afferent) innervation of the cornea is the primary neural input which activates a secretomotor circuit comprising central and peripheral autonomic pathways. The intact neural circuit is also critical for the maintenance of lacrimal gland structure and function. The hypothesis of the proposal is a progressive decline in this neural interaction with age, particularly the decreased corneal sensation, affecting lacrimal gland function. We know that removal of the neural interaction by denervation resulted in structural and functional alteration to the lacrimal gland, and contributes to dry eye disease. Some hints come from gene expression profiling studies which revealed a reduction in the expression of genes relating to the endoplasmic reticulum and secretory apparatus and an increased in expression of genes associated with inflammation and immune regulators. The proposed studies will seek to identify age-specific changes in these patterns of gene expression and theirs transcriptional regulation. We will test the gene response of the lacrimal gland as a function of age by corneal wounding and pharmacologic challenge to determine changes in gene expression profiles. We will utilize the gene expression data to develop a lacrimal gland specific bioinformatics web service.

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