THE EFFECTS OF SUB2 PROTEIN ON GENE SILENCING IN S CEREVISIA

SUB2蛋白对酿酒酵母基因沉默的影响

• 批准号: 7627607
• 负责人: ELAINE E LAHUE
• 金额: $ 4.8万
• 依托单位: UNIVERSITY OF NEBRASKA MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-05-01 至 2008-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7627607
• 关键词: Bacteria; Binding; Biochemical; Biological Assay; Computer Retrieval of Information on Scientific Projects Database; Funding; Gene Silencing; Genes; Grant; Institution; Location; Monitor; Polymerase Chain Reaction; Protein Overexpression; Proteins; Research; Research Personnel; Resources; Role; Source; Standards of Weights and Measures; Time; Transcription Elongation; United States National Institutes of Health; Yeasts; chromatin immunoprecipitation; promoter; research study; telomere; transcription factor

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Determine whether the effect of Sub2p on telomere silencing can be separated from Sub2's role in transcription elongation, using real-time PCR and chromatin immunoprecipitation assays to monitor the location of Sub2. We will assess whether we can locate Sub2 at the promoter of genes that are upregulated when Sub2 is overexpressed. We will also determine whether Sub2 can bind to specific transcription factors using biochemical assays. Three transcription factors have already been verified as Sub2p interactors using co-purification assays in bacteria. However, a more rigid standard would be to recreate this interaction in yeast rather than in bacteria. We are currently pursuing those experiments.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 使用实时PCR和染色质免疫沉淀分析来监测Sub 2的位置，以确定Sub 2 p对端粒沉默的影响是否可以与Sub 2在转录延伸中的作用分开。我们将评估是否可以将Sub 2定位在当Sub 2过表达时上调的基因的启动子处。 我们还将确定是否Sub 2可以结合到特定的转录因子，使用生化测定。 三个转录因子已经被验证为Sub 2 p相互作用使用共纯化测定在细菌中。 然而，一个更严格的标准是在酵母中而不是在细菌中重现这种相互作用。 我们目前正在进行这些实验。