THE EFFECTS OF SUB2 PROTEIN ON GENE SILENCING IN S CEREVISIA

SUB2蛋白对酿酒酵母基因沉默的影响

• 批准号: 7381522
• 负责人: ELAINE E LAHUE
• 金额: $ 4.95万
• 依托单位: UNIVERSITY OF NEBRASKA MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-05-01 至 2007-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7381522
• 关键词: EFFECTS; SUB2; PROTEIN; GENE; SILENCING

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Determine whether the effect of Sub2p on telomere silencing can be separated from Sub2's role in transcription elongation, using real-time PCR and chromatin immunoprecipitation assays to monitor the location of Sub2. We will assess whether we can locate Sub2 at the promoter of genes that are upregulated when Sub2 is overexpressed. We will also determine whether Sub2 can bind to specific transcription factors using biochemical assays. Three transcription factors have already been verified as Sub2p interactors using co-purification assays in bacteria. However, a more rigid standard would be to recreate this interaction in yeast rather than in bacteria. We are currently pursuing those experiments.

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。通过实时荧光定量聚合酶链式反应和染色质免疫沉淀法对端粒沉默的检测，确定端粒沉默是否可以与S在转录延伸中的作用分开。我们将评估是否能够将Sub2定位在当Sub2过度表达时上调的基因的启动子上。我们还将通过生化分析来确定Sub2是否可以与特定的转录因子结合。三个转录因子已经在细菌中通过共纯化实验被证实为Sub2p相互作用因子。然而，一个更严格的标准是在酵母而不是细菌中重建这种相互作用。我们目前正在进行这些实验。