THE EFFECTS OF SUB2 PROTEIN ON GENE SILENCING IN S CEREVISIA

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This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Determine whether the effect of Sub2p on telomere silencing can be separated from Sub2's role in transcription elongation, using real-time PCR and chromatin immunoprecipitation assays to monitor the location of Sub2. We will assess whether we can locate Sub2 at the promoter of genes that are upregulated when Sub2 is overexpressed. We will also determine whether Sub2 can bind to specific transcription factors using biochemical assays. Three transcription factors have already been verified as Sub2p interactors using co-purification assays in bacteria. However, a more rigid standard would be to recreate this interaction in yeast rather than in bacteria. We are currently pursuing those experiments.