MISSISSIPPI COBRE: P4: ID OF REWARDING CONSTITUENTS IN BOTANICAL EXTRACTS

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 该项目的首要目标是开发一个标准化的协议，使用传统的体内生物测定，以促进化学复杂的植物产品的筛选，有效地识别植物成分，具有奖励或厌恶的属性，可能最终限制其作为治疗剂的使用的目标。该项目将利用国家天然产品研究中心的天然产品科学家的专业知识，提供筛选所需的植物提取物和馏分。 第一个目的是使用条件性位置偏好/回避（CPP/CPA）范例作为体内生物测定筛选植物提取物，馏分和成分和/或半合成的单一实体化合物的推定的增强或厌恶的属性。 第二个目标是结合和验证使用多元回归/相关（MRC）设计和分析，以查明植物提取物或馏分中的“活性”，而不需要筛选每一个单独的分离成分。 在该统计设计中，CPP/CPA评分作为预测变量，一系列植物提取物中的不同成分水平作为标准变量。 由此产生的分析将预测哪些成分或成分负责植物内的奖励/厌恶属性。 希望这样的MRC设计和分析筛选方案推广到其他体内筛选测定（例如，焦虑、抑郁、镇痛）中寻找植物产品中含有的推定治疗成分。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The overarching goal of this project is to develop a standardized protocol using a traditional in vivo bioassay to facilitate the screening of chemically complex botanical products with the goal of efficiently identifying plant constituents that possess rewarding or aversive properties that may ultimately limit their use as therapeutic agents. This project will utilize the expertise of natural product scientists at the National Center for Natural Products Research in providing the needed plant extracts and fractions for screening. The first aim is to use the conditioned place preference/avoidance (CPP/CPA) paradigm as an in vivo bioassay to screen botanical extracts, fractions and constituents and/or semisynthetic single entity compounds for putative reinforcing or aversive properties. The second aim is to incorporate and validate the use of multiple regression/correlation (MRC) design and analysis to pinpoint "activity" in a plant extract or fraction without the need to screen every single isolated constituent. In this statistical design, the CPP/CPA score serves as the predictor variable and varying constituent levels in a series of plant extracts as the criterion variable. The resulting analyses will predict which constituent or constituents are responsible for the rewarding/aversive properties within a plant. It is hoped that such an MRC design and analysis screening protocol generalizes to other in vivo screening assays (e.g., anxiety, depression, analgesia) in their search for putative therapeutic constituents contained in botanical products.