MISSISSIPPI COBRE: P4: ID OF REWARDING CONSTITUENTS IN BOTANICAL EXTRACTS

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 该项目的总体目标是开发一种使用传统的体内生物测定的标准化方案，以促进筛选化学上复杂的植物产品，目标是有效地识别具有奖励或厌恶特性的植物成分，这些特性可能最终限制其作为治疗剂的使用。该项目将利用国家天然产品研究中心天然产品科学家的专业知识，为筛选提供所需的植物提取物和级分。第一个目标是使用条件性位置偏爱/回避(CPP/CPA)范式作为体内生物测定来筛选植物提取物、组分和成分和/或半合成的单一实体化合物的可能的增强或厌恶特性。第二个目标是结合和验证多元回归/相关(MRC)设计和分析的使用，以精确定位植物提取物或组分中的“活性”，而不需要筛选每一个单独的成分。在这个统计设计中，CPP/CPA得分作为预测变量，而一系列植物提取物中不同的成分水平作为标准变量。由此产生的分析将预测哪一种或哪些成分对植物内的奖励/厌恶特性负责。希望这种MRC设计和分析筛选方案可推广到其他体内筛选分析方法(如焦虑、抑郁、止痛)，以寻找植物产品中可能包含的治疗成分。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The overarching goal of this project is to develop a standardized protocol using a traditional in vivo bioassay to facilitate the screening of chemically complex botanical products with the goal of efficiently identifying plant constituents that possess rewarding or aversive properties that may ultimately limit their use as therapeutic agents. This project will utilize the expertise of natural product scientists at the National Center for Natural Products Research in providing the needed plant extracts and fractions for screening. The first aim is to use the conditioned place preference/avoidance (CPP/CPA) paradigm as an in vivo bioassay to screen botanical extracts, fractions and constituents and/or semisynthetic single entity compounds for putative reinforcing or aversive properties. The second aim is to incorporate and validate the use of multiple regression/correlation (MRC) design and analysis to pinpoint "activity" in a plant extract or fraction without the need to screen every single isolated constituent. In this statistical design, the CPP/CPA score serves as the predictor variable and varying constituent levels in a series of plant extracts as the criterion variable. The resulting analyses will predict which constituent or constituents are responsible for the rewarding/aversive properties within a plant. It is hoped that such an MRC design and analysis screening protocol generalizes to other in vivo screening assays (e.g., anxiety, depression, analgesia) in their search for putative therapeutic constituents contained in botanical products.