The Effects of Developmental Hearing Loss on Inhibitory Short-term Plasticity

发育性听力损失对抑制性短期可塑性的影响

• 批准号: 7670354
• 负责人: ANNE E TAKESIAN
• 金额: $ 2.61万
• 依托单位: NEW YORK UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-09-01 至 2010-06-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7670354
• 关键词: Acoustics; Action Potentials; Agonist; Animals; Anus; Auditory; Auditory area; Behavioral; Cell physiology; Cells; Complex; Cues; Data; Development; Environment; Event; Excitatory Synapse; Figs - dietary; Hearing; Hearing Impaired Persons; Human; Impairment; Inhibitory Synapse; Knowledge; Language; Learning; Link; Measures; Mediating; Music; Neurons; Pattern; Perception; Pharmacology; Physiologic pulse; Preparation; Probability; Process; Production; Property; Protocols documentation; Receptor Signaling; Role; Series; Signal Transduction; Slice; Speech; Stimulus; Synapses; Time; Training; Whole-Cell Recordings; Work; deafness; design; experience; hearing impairment; language perception; postnatal; postsynaptic; presynaptic; prevent; receptor; research study; response; sound; synaptic inhibition; transmission process; treatment strategy

DESCRIPTION (provided by applicant): Humans who have suffered from periods of hearing loss during development can experience permanent debilitating impairments in sound and language perception! These impairments include deficits in spectrotemporal processing. Inhibitory circuits in the auditory cortex are vulnerable to hearing loss and may be critical for spectrotemporal processing. Specifically, inhibitory transmission displays short-term plasticity which may contribute to the encoding of temporal patterns of acoustic stimuli. My preliminary data suggests that inhibitory short-term plasticity is altered by deafness. The objective of this application is to study how deafness may impact normal development of inhibitory short-term plasticity and how these changes may alter efficacy and timing of auditory cortex discharge patterns. A series of whole-cell experiments in neurons of the'auditory cortex are specifically designed to characterize (1) the normal development of inhibitory short-term plasticity (2) the impact of deafness on inhibitory short- term plasticity (3) the effects of inhibitory short-term plasticity on discharge properties. To characterize development of inhibitory short-term plasticity in normal and deaf animals, paired and multiple-pulse stimuli will be used to evoke trains of inhibitory synaptic events. Pharmacological manipulations, particularly targeting pre and postsynaptic GABAB receptors, will be used to uncover the mechanisms underlying normal developmental short-term plasticity and the changes following deafness. Finally, to understand the functional consequences of these changes, the effects of inhibitory short-term plasticity on spike efficacy and timing will be evaluated by integrating synaptic inhibition with neuronal spiking. Relevance: These experiments will assess whether changes in temporal responses of inhibitory synapses may partially explain auditory processing deficits following hearing loss, including impairments in speech production and perception. Particularly due to pur extensive knowledge of GABAB receptor pharmacology, revealing the involvement of these receptors in temporal responses will broaden our strategies to prevent and alleviate behavioral deficits associated with hearing loss.

描述（由申请人提供）：在发育过程中遭受听力损失的人可能会在声音和语言感知方面经历永久性的衰弱性障碍！这些损伤包括频谱时间处理的缺陷。听觉皮层中的抑制回路容易受到听力损失的影响，并且可能对频谱时间处理至关重要。具体而言，抑制性传输显示短期可塑性，这可能有助于编码的时间模式的声刺激。我的初步数据表明，抑制性短期可塑性被耳聋改变。本申请的目的是研究耳聋如何影响抑制性短期可塑性的正常发展，以及这些变化如何改变听觉皮层放电模式的功效和时间。本研究采用全细胞实验的方法，研究了听觉皮层神经元抑制性短时程可塑性的正常发育过程，耳聋对抑制性短时程可塑性的影响，以及抑制性短时程可塑性对听觉皮层神经元放电特性的影响。为了表征正常和耳聋动物中抑制性短期可塑性的发展，将使用成对和多脉冲刺激来诱发抑制性突触事件的序列。药理学操作，特别是针对突触前和突触后GABAB受体，将被用来揭示正常发育的短期可塑性和耳聋后的变化的机制。最后，为了了解这些变化的功能后果，抑制性短期可塑性对尖峰效力和时间的影响将通过整合突触抑制与神经元尖峰来评估。相关性：这些实验将评估抑制性突触的时间反应的变化是否可以部分解释听力损失后的听觉处理缺陷，包括言语产生和感知的障碍。特别是由于我们对GABAB受体药理学的广泛了解，揭示这些受体在时间反应中的参与将拓宽我们预防和减轻与听力损失相关的行为缺陷的策略。