Neuroprotection Effects of Sex Steroids in EAE

性类固醇对 EAE 的神经保护作用

• 批准号: 7575245
• 负责人: Halina Offner
• 金额: $ 31.47万
• 依托单位: OREGON HEALTH & SCIENCE UNIVERSITY
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7575245
• 关键词: Age; Aging; Agonist; Androgen Receptor; Androgens; Animal Model; Antigen-Presenting Cells; Antigens; Aromatase; Aromatase Inhibitors; Astrocytes; B-Lymphocytes; Bone Marrow; Bone Marrow Cells; Castration; Cells; Central Nervous System Diseases; Cerebral Ischemia; Chemosensitization; Chronic; Clinical; Complement; Cytochrome P450; Data; Dendritic Cells; Development; Disease; Disease susceptibility; Dose; Encephalomyelitis; Enzymes; Estradiol; Estrogens; Experimental Autoimmune Encephalomyelitis; Fadrozole; Flutamide; Gonadal Steroid Hormones; Hormones; Immune; Indium; Inflammatory; Ischemia; Knock-out; L-3; Life; Mediating; Microglia; Modeling; Multiple Sclerosis; Mus; Myelin; Myelin Basic Proteins; Neurons; Oligodendroglia; Oxidoreductase; Pathology; Pathway interactions; Patients; Placebos; Production; Proteins; Proteolipids; Purpose; Receptor Signaling; Refractory; Relapse; Reporting; Research Personnel; Residual state; Resistance; Severities; Signal Transduction; Spleen; Stanolone; Supplementation; T-Lymphocyte; Testing; Testosterone; Thinking; age related; cell type; chemokine; chemokine receptor; cytokine; human disease; immune function; insight; interest; macrophage; male; men; middle age; mouse model; neuroprotection; oligodendrocyte-myelin glycoprotein; programs; protective effect; receptor; research study; young adult

Low doses of sex steroids, including 17-f5 estradiol (E2) and testosterone (T4), confer protection against clinical and histological experimental autoimmune encephalomyelitis (EAE), an animal model for the human disease, multiple sclerosis (MS). We hypothesize that the CNSneuroprotective effects of both sex steroids on EAE are mediated through both immune and non-immune cells. E2 may contribute to EAE resistance by influencingthe development and function of potentially pathogenic T cells specific for myelin antigens, as well as regulatory T cells, including the newly described CD4+CD25+ Treg cells, which might modify the course of disease. Alternatively, the primary effects of E2 might be on macrophages, dendritic cells, or CNS cells. We found that E2 treatment inhibited encephalitogenic activity of myelin-specific T cells, reduced cytokines (particularly TNF-a) and chemokines/receptors in the CNS and spleen, and inhibited recruitment of inflammatory cells into the CNS. Recently, we demonstrated that ERKO mice lackingEsrl (a receptor for E2) but not BERKO mice lacking Esr2 (P receptor for E2) were refractory to E2-mediated inhibition of chronic EAE, thus implicatingEsrl in E2-mediated protection. In Aim 1, we will determine if immune or non-immune cells mediate the neuroprotective effects of E2 in chronic (C57BL/6) and relapsing (SJL) models of EAE. In Aim 2, we will pursue promising preliminary data indicating that E2 may potentiate the regulatory activity of CD4+CD25+ T cells that may also function through Esrl. T4 may also provide protection from EAE, as we have previously shown. Castration worsens EAE in our young male mouse model, while exogenous T4 treatment reduces disease. We hypothesize that T4 provides protection because it is converted to E2 via the P450 aromatase, and this effect is most pronounced in middle-aged males. Alternatively, T4 may act by direct signaling through the androgen receptor (AR). In Aim 3, we will test the hypothesis that exogenous T4 treatment provides protection against EAE through enzymatic conversion that is age dependent. Results obtained from this project will selectively identify E2-responsive cell types in EAE and determine if estrogen and testosterone protection against EAE utilize overlapping mechanisms, thus providing a direct comparison with neuroprotective mechanisms of T4 in cerebral ischemia (Project 2). Our studies may be useful in predicting how testosterones might benefit middle- aged patients with MS.

低剂量性类固醇，包括17-f5雌二醇(E2)和睾酮(T4)，提供了对临床和 组织学实验性自身免疫性脑脊髓炎(EAE)，一种人类疾病的动物模型， 硬化症(MS)。我们推测，两性类固醇对EAE的神经保护作用是通过 包括免疫细胞和非免疫细胞。E2可能通过影响EAE的发育和功能而参与EAE的抵抗 针对髓鞘抗原的潜在致病T细胞以及调节性T细胞，包括新生的 描述了可能改变病程的CD4+CD25+Treg细胞。或者，雌二醇的主要作用 可能在巨噬细胞、树突状细胞或中枢神经系统细胞上。我们发现雌二醇组抑制了脑源性活动。 髓鞘特异性T细胞，中枢神经系统和脾中细胞因子(尤其是肿瘤坏死因子-α)和趋化因子/受体减少， 并抑制炎症细胞向中枢神经系统的募集。最近，我们证明了Erko小鼠缺乏Esrl (E_2受体)而不是缺乏ESR2(E_2的P受体)的BERKO小鼠对E_2介导的抑制作用不敏感。 慢性EAE，因此暗示ESR1参与了E2介导的保护。在目标1中，我们将确定免疫或非免疫 细胞介导E2对慢性(C57BL/6)和复发性(SJL)EAE模型的神经保护作用。在目标2中，我们 将寻求有希望的初步数据，表明E2可能增强CD4+CD25+T细胞的调节活性 这也可能通过ESRL发挥作用。T4还可以提供对EAE的保护，正如我们之前所展示的那样。 在我们的年轻雄性小鼠模型中，去势会恶化EAE，而外源性T4治疗则减少了疾病。我们 假设T4提供保护是因为它通过P450芳香酶转化为E2，这种效应是 在中年男性中最为明显。或者，T4可以通过雄激素直接发出信号来发挥作用 受体(AR)。在目标3中，我们将检验外源性T4治疗提供保护的假设。 EAE通过酶转化，这是年龄依赖的。从这个项目中获得的结果将有选择地 确定EAE中的E2反应细胞类型，并确定雌激素和睾酮是否对EAE具有保护作用 利用重叠机制，从而与T4的神经保护机制进行直接比较 脑缺血(项目2)。我们的研究可能有助于预测睾酮如何对中年女性有益。 老年多发性硬化患者。