Point Source Ozonation to Minimize Antibiotic Resistance

描述（由申请人提供）：在这个第二阶段项目中，BlueInGreen，LLC将建立一个超饱和溶解臭氧（HYDOZTM）装置，在阿肯色州斯普林代尔的斯普林代尔污水处理设施（SWWTF）进行大型中试研究。此外，BlueInGreen将与阿肯色州大学合作，在实验室规模和中试规模研究中测量HYDOZ臭氧化去除废水中难降解微生物和稳定化学污染物的能力。第一阶段的结果表明，提高效率和有效性的HYDOZ臭氧处理在实验室规模相比，传统的臭氧化系统。第二阶段的研究将集中在稳定的污染物和难降解的微生物，不能从废水中去除使用标准的氯基消毒。我们将证明，使用HYDOZ在集中式废水处理设施中处理废水是一种经济和技术上可行的方法，可以防止药物残留物和耐药微生物释放到环境中。该项目直接涉及国家环境健康科学研究所的使命，即减少环境对人类疾病和功能障碍的负担，并将展示HYDOZ臭氧化废水的功效和效率。第二阶段的具体目标是：“目标1：比较HYDOZ与标准消毒/去污技术（蓝绿）的成本和功效。里程碑1：HYDOZ系统的资本和运行成本上级氯基消毒系统，并且基于标准流出物质量参数，HYDOZ系统上级用于处理废水流出物的氯基消毒系统。目标2：研究HYDOZ对废水中一系列化学污染物的破坏作用（蓝绿）。里程碑二：HYDOZ臭氧化系统将使废水中至少六种化学污染物的浓度降低至少90%。“目的3：研究HYDOZ对废水中质粒和难降解微生物的破坏（阿肯色州大学）。里程碑3：HYDOZ臭氧化系统将使废水排放物中的质粒和难降解微生物的浓度降低至少90%。 公共卫生相关性：该项目的目标是使用超饱和溶解臭氧（HYDOZTM）系统去除废水中稳定的化学/药物残留物和难降解微生物。成功减少水环境中这些污染物的出现将减少对生态系统和人类的长期影响，并减少抗生素耐药微生物的传播。该器械的广泛使用将大大有助于减少人类生态系统中的抗生素耐药性病原体，并有助于维持医学上重要的抗生素的疗效。

DESCRIPTION (provided by applicant): In this Phase II project, BlueInGreen, LLC will build a hypersaturated dissolved ozone (HYDOZTM) unit to deploy in a large pilot-scale study at Springdale Wastewater Treatment Facility (SWWTF) in Springdale, Arkansas. Furthermore, BlueInGreen will collaborate with the University of Arkansas to measure the ability of HYDOZ ozonation to remove refractory microbes and stable chemical contaminants from wastewater effluent in both lab-scale and pilot- scale studies. Phase I results demonstrated the increased efficiency and effectiveness of HYDOZ ozone treatment at bench scale compared to conventional ozonation systems. Phase II research will focus on stable contaminants and refractory microbes that are not removed from wastewater using standard chlorine-based disinfection. We will show that the use of the HYDOZ to treat wastewater at centralized wastewater treatment facilities is a viable method economically and technologically to prevent the release of pharmaceutical residuals and resistant microbes to the environment. This project directly addresses the mission of the National Institute of Environmental Health Sciences to reduce the burden of human disease and dysfunction from the environment and will demonstrate both the efficacy and efficiency of HYDOZ ozonation of wastewater effluent. The specific Phase II objectives are: " Objective 1: Compare cost and efficacy of the HYDOZ to standard disinfection/decontamination technology (BlueInGreen). Milestone 1: The capitol and operating cost of HYDOZ systems is superior to chlorine-based disinfection systems, and based on standard effluent quality parameters, the HYDOZ systems are superior to chlorine-based disinfection systems for treatment of wastewater effluent. " Objective 2: Study HYDOZ destruction of a range of chemical contaminants in wastewater effluent (BlueInGreen). Milestone 2: The HYDOZ ozonation systems will cause a minimum 90% reduction in concentration of at least six chemical contaminants from wastewater effluent. " Objective 3: Study HYDOZ destruction of plasmids and refractory microbes in wastewater effluent (University of Arkansas). Milestone 3: The HYDOZ ozonation systems will cause a minimum 90% reduction in concentration of plasmids and refractory microbes from wastewater effluent. PUBLIC HEALTH RELEVANCE: The goal of this project is to use the hypersaturated dissolved ozone (HYDOZTM) system to remove stable chemical/pharmaceutical residuals and refractory microbes from wastewater. Successful reduction of the occurrence of these contaminants in the aqueous environment will reduce long-term impacts on ecosystems and humans, and reduce the spread of antibiotic resistant microbes. The widespread use of this device will significantly contribute to the reduction of antibiotic resistant pathogens in the human ecosystem and help maintain the efficacy of medically important antibiotics.