DETECTION OF ALZHEIMER'S DISEASE USING A POLYMER NANOWIRE-BASED BIOSENSOR

使用基于聚合物纳米线的生物传感器检测阿尔茨海默病

• 批准号: 7596948
• 负责人: Maksudul Alam
• 金额: $ 19.95万
• 依托单位: INNOSENSE, LLC
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-04-01 至 2010-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7596948
• 关键词: Address; Affect; Alzheimer disease detection; Alzheimer disease screening; Alzheimer' s Disease; American; Amides; Amyloid; Antibodies; Antigen Targeting; Antigens; Area; Autopsy; Binding; Biochemistry; Biocompatible; Biological Markers; Biosensor; Boxing; Brain; Caliber; California; Caregivers; Caring; Characteristics; Chemicals; Clinical; Dementia; Deposition; Detection; Development; Devices; Diagnostic; Disease; Disease Marker; Early Diagnosis; Early treatment; Elderly; Electrical Resistance; Electrodes; Electronics; Electroplating; Ensure; Exposure to; Fertilization; Film; Germ Cells; Goals; Growth; Human; Individual; Knowledge; Laboratory Diagnosis; Left; Length; Ligand Binding; Ligands; Link; Measures; Mediating; Methods; Microelectrodes; Modeling; Modification; Molecular; Molecular Biology; Monitor; Neurodegenerative Disorders; Noise; One-Step dentin bonding system; Oxidation-Reduction; Pathogenesis; Pattern; Peptides; Performance; Phase; Polymers; Principal Investigator; Process; Property; Protocols documentation; Quality of life; Research; Resistance; Role; Sampling; Sea Urchins; Senile Plaques; Signal Transduction; Silicon; Solutions; Specificity; Surface; Symptoms; System; Techniques; Technology; Temperature; Time; Universities; Work; antigen antibody binding; artist; base; clinical Diagnosis; computerized data processing; cost; cost effective; covalent bond; design; detector; disease diagnosis; electrical property; end of life; experience; improved; innovation; meetings; monolayer; monomer; nano; nanoscale; nanowire; plaque lesion; polyaniline; polymerization; polypyrrole; professor; prototype; response; self assembly; sensor; solid state; success; tool

DESCRIPTION (provided by applicant): InnoSense LLC (ISL) submits this revised application with the intent of developing a cost-effective and highly sensitive polymer nanowire-based electronic biosensor (PNEBD) for the early screening of Alzheimer's disease (AD). This device is expected to have potentially significant implications in the early treatment and possible delay of debilitating symptoms associated with this disease. The Phase I project is focused on the development and verification of a high-throughput device based on silicon photolithography processes and template-free electrodeposition of functionalized conductive polymers between narrow-gap electrode junctions. Six tasks have been proposed to establish the Phase I feasibility of detecting amyloid-¿ derived diffusible ligand (ADDL) antigen, a biomarker for AD, using amyloid-¿ (A¿) oligomer specific antibody to ADDLs. Current evidence strongly suggests a central role for amyloid-¿ (A¿) in the pathogenesis of AD. At the present time there is no definite clinical diagnosis for AD other than autopsy. It has been demonstrated that ADDLs are present at significantly elevated levels in the autopsied brain samples of humans with AD. This association of ADDLs with AD suggests that the PNEBD device could provide a definitive molecular basis for the laboratory diagnosis of AD. For ensuring success, a highly skilled research team, including an expert on AD, has been assembled to execute the project. Development of ultrasensitive detection methods for early screening of Alzheimer's disease (AD) is an important issue in clinical disease diagnosis and mechanistic understanding of Alzheimer's disease (AD). AD is a progressive neurodegenerative disease for which there is neither a cure nor an early diagnostic device. It is the leading cause of dementia in people over age 65 affecting over four million Americans. Despite its utmost importance, no ultrasensitive technologies have been developed for clinical lab diagnostic of AD. InnoSense LLC aims to fill this void by developing a cost-effective and highly-sensitive polymer nanowire-based electronic biosensor device (PNEBD). This device will utilize electrochemically grown polymer nanowires at micro- to nanoscale patterned electrode junctions for detecting amyloid-¿ derived diffusible ligands (ADDLs). ADDL has been established as a biomarker for AD. The purpose of using an electrochemical method is to grow template-free, low-cost and high quality polymer nanowires on to the electrode junctions. This direct, one-step fabrication technique is scalable. This project will advance our knowledge on the detection of Alzheimer's disease.

描述（由应用程序提供）：Innosense LLC（ISL）提交了此修订后的应用，目的是开发一种基于成本效益且高度敏感的聚合物电子生物传感器（PNEBD），以早日筛查阿尔茨海默氏病（AD）。预计该设备对早期治疗以及与该疾病相关的症状的可能延迟具有潜在的显着意义。已经提出了I阶段项目的六个任务，以确定检测淀粉样蛋白的可行性的可行性，该淀粉样蛋白可扩散的配体（ADDL）抗原是AD的生物标志物，用于AD的生物标志物，使用淀粉样蛋白（a。）降低了ADDL的低聚物特异性抗体。当前的证据强烈表明淀粉样蛋白（A。）在AD发病机理中起着核心作用。目前，除尸检以外，没有其他AD的临床诊断。已经证明，在具有AD的人类的尸体脑样本中，ADDL存在于显着升高的水平。 ADDL与AD的这种关联表明，PNEBD设备可以为AD的实验室诊断提供明确的分子基础。为了确保成功，包括广告专家在内的一支高技能的研究团队已被组装以执行该项目。早期筛查阿尔茨海默氏病（AD）的超敏感检测方法的发展是临床疾病诊断和对阿尔茨海默氏病（AD）的机械理解（AD）的重要问题。 AD是一种进行性神经退行性疾病，既没有治愈方法也没有早期诊断装置。这是65岁以上人群痴呆症的主要原因，影响了超过400万美国人。尽管最重要的是，尚未开发出用于AD的临床实验室诊断的超敏感技术。 Innosense LLC的目的是通过开发基于成本效益且高敏感的聚合物电子生物传感器设备（PNEBD）来填补这一空白。该设备将利用微化学生长的聚合物纳米线在微观到纳米级图案的电极连接处检测淀粉样蛋白 - 衍生的扩散配体（ADDLS）。 ADDL已成为AD的生物标志物。使用电化学方法的目的是将无模板，低成本和高质量的聚合物纳米线生长到电极连接。这种直接的一步制造技术是可扩展的。该项目将促进我们对检测阿尔茨海默氏病的知识。