DETECTION OF ALZHEIMER'S DISEASE USING A POLYMER NANOWIRE-BASED BIOSENSOR

使用基于聚合物纳米线的生物传感器检测阿尔茨海默病

• 批准号: 7596948
• 负责人: Maksudul Alam
• 金额: $ 19.95万
• 依托单位: INNOSENSE, LLC
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-04-01 至 2010-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7596948
• 关键词: Address; Affect; Alzheimer disease detection; Alzheimer disease screening; Alzheimer' s Disease; American; Amides; Amyloid; Antibodies; Antigen Targeting; Antigens; Area; Autopsy; Binding; Biochemistry; Biocompatible; Biological Markers; Biosensor; Boxing; Brain; Caliber; California; Caregivers; Caring; Characteristics; Chemicals; Clinical; Dementia; Deposition; Detection; Development; Devices; Diagnostic; Disease; Disease Marker; Early Diagnosis; Early treatment; Elderly; Electrical Resistance; Electrodes; Electronics; Electroplating; Ensure; Exposure to; Fertilization; Film; Germ Cells; Goals; Growth; Human; Individual; Knowledge; Laboratory Diagnosis; Left; Length; Ligand Binding; Ligands; Link; Measures; Mediating; Methods; Microelectrodes; Modeling; Modification; Molecular; Molecular Biology; Monitor; Neurodegenerative Disorders; Noise; One-Step dentin bonding system; Oxidation-Reduction; Pathogenesis; Pattern; Peptides; Performance; Phase; Polymers; Principal Investigator; Process; Property; Protocols documentation; Quality of life; Research; Resistance; Role; Sampling; Sea Urchins; Senile Plaques; Signal Transduction; Silicon; Solutions; Specificity; Surface; Symptoms; System; Techniques; Technology; Temperature; Time; Universities; Work; antigen antibody binding; artist; base; clinical Diagnosis; computerized data processing; cost; cost effective; covalent bond; design; detector; disease diagnosis; electrical property; end of life; experience; improved; innovation; meetings; monolayer; monomer; nano; nanoscale; nanowire; plaque lesion; polyaniline; polymerization; polypyrrole; professor; prototype; response; self assembly; sensor; solid state; success; tool

DESCRIPTION (provided by applicant): InnoSense LLC (ISL) submits this revised application with the intent of developing a cost-effective and highly sensitive polymer nanowire-based electronic biosensor (PNEBD) for the early screening of Alzheimer's disease (AD). This device is expected to have potentially significant implications in the early treatment and possible delay of debilitating symptoms associated with this disease. The Phase I project is focused on the development and verification of a high-throughput device based on silicon photolithography processes and template-free electrodeposition of functionalized conductive polymers between narrow-gap electrode junctions. Six tasks have been proposed to establish the Phase I feasibility of detecting amyloid-¿ derived diffusible ligand (ADDL) antigen, a biomarker for AD, using amyloid-¿ (A¿) oligomer specific antibody to ADDLs. Current evidence strongly suggests a central role for amyloid-¿ (A¿) in the pathogenesis of AD. At the present time there is no definite clinical diagnosis for AD other than autopsy. It has been demonstrated that ADDLs are present at significantly elevated levels in the autopsied brain samples of humans with AD. This association of ADDLs with AD suggests that the PNEBD device could provide a definitive molecular basis for the laboratory diagnosis of AD. For ensuring success, a highly skilled research team, including an expert on AD, has been assembled to execute the project. Development of ultrasensitive detection methods for early screening of Alzheimer's disease (AD) is an important issue in clinical disease diagnosis and mechanistic understanding of Alzheimer's disease (AD). AD is a progressive neurodegenerative disease for which there is neither a cure nor an early diagnostic device. It is the leading cause of dementia in people over age 65 affecting over four million Americans. Despite its utmost importance, no ultrasensitive technologies have been developed for clinical lab diagnostic of AD. InnoSense LLC aims to fill this void by developing a cost-effective and highly-sensitive polymer nanowire-based electronic biosensor device (PNEBD). This device will utilize electrochemically grown polymer nanowires at micro- to nanoscale patterned electrode junctions for detecting amyloid-¿ derived diffusible ligands (ADDLs). ADDL has been established as a biomarker for AD. The purpose of using an electrochemical method is to grow template-free, low-cost and high quality polymer nanowires on to the electrode junctions. This direct, one-step fabrication technique is scalable. This project will advance our knowledge on the detection of Alzheimer's disease.

描述（由申请人提供）：InnoSense LLC （ISL）提交此修订申请，旨在开发一种具有成本效益且高灵敏度的聚合物纳米线电子生物传感器（PNEBD），用于阿尔茨海默病（AD）的早期筛查。该装置有望在早期治疗和可能延缓与该疾病相关的衰弱症状方面具有潜在的重要意义。第一期项目的重点是开发和验证基于硅光刻工艺和窄间隙电极结之间功能化导电聚合物的无模板电沉积的高通量设备。利用淀粉样蛋白- (a)寡聚物特异性抗体检测AD生物标志物淀粉样蛋白衍生扩散配体（ADDL）抗原的I期可行性，提出了六个任务。目前的证据强烈表明淀粉样蛋白-¿（a¿）在阿尔茨海默病的发病机制中的核心作用。目前，除了尸体解剖外，对阿尔茨海默病没有明确的临床诊断。已经证明，在AD患者的尸检脑样本中，addl的水平显著升高。adls与AD的关联表明PNEBD装置可以为AD的实验室诊断提供明确的分子基础。为了确保成功，我们组建了一支高技能的研究团队，包括一名AD专家，来执行这个项目。开发用于阿尔茨海默病（AD）早期筛查的超灵敏检测方法是临床疾病诊断和了解阿尔茨海默病（AD）机制的重要课题。阿尔茨海默病是一种进行性神经退行性疾病，目前既没有治愈方法，也没有早期诊断设备。它是65岁以上人群痴呆症的主要原因，影响了400多万美国人。尽管它非常重要，但目前还没有开发出用于临床实验室诊断AD的超灵敏技术。InnoSense LLC旨在通过开发一种具有成本效益和高灵敏度的基于聚合物纳米线的电子生物传感器设备（PNEBD）来填补这一空白。该装置将利用电化学生长的聚合物纳米线在微到纳米尺度的图案电极连接处检测淀粉样蛋白衍生的扩散配体（ADDLs）。ADDL已被确定为AD的生物标志物。使用电化学方法的目的是在电极结上生长无模板、低成本和高质量的聚合物纳米线。这种直接的一步制造技术是可扩展的。这个项目将提高我们对阿尔茨海默病检测的认识。