Spectrally resolved tomographic small-animal fluorescence imager

光谱分辨断层小动物荧光成像仪

基本信息

批准号：
7686997
负责人：
RICHARD M. LEVENSON
金额：
$ 87.57万
依托单位：
CAMBRIDGE RESEARCH AND INSTRUMENTATION
依托单位国家：
美国
项目类别：
财政年份：
2007
资助国家：
美国
起止时间：
2007-05-01 至 2011-04-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7686997
关键词：
3-Dimensional Acceleration Address Algorithms American Anesthesia procedures Animal Disease Models Animal Model Animals Atlases Autopsy Biological Models Calibration California Cardiac Cells Chemistry Cherry - dietary Clinical Laboratory Information Systems Collaborations Collection Communities Computer software Containment Custom Data Detection Development Disease Disease model Dyes Electronics Elements Employment Ensure European Evaluation Eye Face Fluorescence Focus Groups Freezing Frozen Sections Generations Glass Goals Heating Height Hour Image Image Analysis Imagery Indium Institutes Joints Label Legal patent Libraries Light Lighting Liquid substance Location Lung Maps Measures Mechanics Methods Modeling Molecular Probes Movement Mus Noise Operative Surgical Procedures Optical Tomography Optics Pattern Performance Pharmacologic Substance Phase Physiology Play Probability Process Property Publications Quantum Dots Reagent Regulation Reporter Reporting Research Resolution Risk Role Safety Sampling Shapes Side Signal Transduction Site Small Animal Imaging Systems Small Business Innovation Research Grant Software Tools Software Validation Source Speed Structure Submersion Surface System Techniques Testing Time Tissue Model Tissues Training Universities Validation Variant Vascular Diseases Washington Work aged base design digital drug discovery experience flexibility fluorophore imaging modality improved in vivo indexing instrument interest lens light emission lung imaging malignant breast neoplasm meetings millimeter mouse model novel optical imaging performance tests prototype reconstruction research study sensor simulation tool

项目摘要

DESCRIPTION (provided by applicant): This is a Fast-Track proposal to develop a multispectral optical tomography small-animal imaging system for use by the biomedical and pharmaceutical research communities. It will combine the experience CRI has acquired in multispectral, planar small-animal imaging (through its development of the Maestro(tm) system) with novel hyperspectral optical tomography algorithms and will be realized using a unique optical design. The primary aim of this proposal is to refine 3-dimensional (3D) localization of point and extended fluorescent sources within small animals. Tomographic approaches are essential for achieving truly quantitative, depth- resolved imaging. Partners in this project include leaders in optical design, algorithmic development, dye chemistry, advanced small-animal models of disease, and directors of animal imaging facilities. Key properties include: 1) the use of a spectrally tunable filter with unmatched flexibility for multiplexed fluorophore signal detection in the presence of significant autofluorescence; 2) a novel optic that enables multi- view detection; 3) a customized multispectral fluorescent label strategy; and 4) no requirement for camera or subject movement, compression of the subject between glass plates, or submersion of the subject in index- and scatter-matching fluids. Phase I will accomplish: construction of a first-generation instrument with off-the-shelf optics; radiometric characterization; surface profilometry, and confirmation via simulation that the instrument will accomplish 3D reconstructions with a resolution of 2 millimeters or better. Phase II will be dedicated to building a second- generation version of the instrument with custom, high-efficiency optics, and will add a variety of important algorithmic, computational and reagent components for optimal performance, with an emphasis on quantitative validation. The system will be deployed at several animal imaging facilities. A variety of animal models of disease, including metastatic breast cancer, pulmonary, cardiac and vascular disease will be studied and the performance of the Maestro-3D compared to competing optical and non-optical imaging modalities. In addition, CRI will work on methods to accelerate time consuming tomographic reconstructions. Finally, easy-to-use software for control, display, quantitation and integration into laboratory information systems will be developed.

描述（由申请人提供）：这是一项快速提案，旨在开发一个多光谱光学层析成像小动物成像系统，以供生物医学和药物研究社区使用。它将结合CRI在多光谱的，平面的小动物成像中获得的体验（通过其大师（TM）系统的开发）与新型的高光谱光学层析成像算法，并将使用独特的光学设计实现。该提案的主要目的是完善小动物中点和荧光源的3维（3D）定位。层析成像方法对于实现真正定量，深度分辨的成像至关重要。该项目的合作伙伴包括光学设计领域的领导者，算法开发，染料化学，高级小动物疾病模型以及动物成像设施的董事。关键属性包括：1）在存在明显的自动荧光的情况下，使用具有无与伦比的灵活性的频谱可调滤波器用于多路复用荧光团信号检测； 2）一种可实现多视图检测的新型视频； 3）定制的多光谱荧光标签策略； 4）不需要摄像机或受试者运动，玻璃板之间的受试者压缩或在索引和散射匹配的液体中浸没。第一阶段将完成：建造具有现成光学的第一代仪器；辐射表征；表面经理仪和通过模拟确认，该仪器将完成3D重建，分辨率为2毫米或更高。第二阶段将致力于使用自定义，高效率光学器件构建仪器的第二代版本，并将添加各种重要的算法，计算和试剂组件，以实现最佳性能，并强调定量验证。该系统将部署在几个动物成像设施中。将研究各种动物模型，包括转移性乳腺癌，肺部，心脏和血管疾病，并且与竞争的光学和非光学成像方式相比，Maestro-3D的性能。此外，CRI将致力于加速耗时层析成像重建的方法。最后，将开发用于控制，显示，定量和集成到实验室信息系统中的易于使用的软件。