Spectrally resolved tomographic small-animal fluorescence imager

光谱分辨断层小动物荧光成像仪

• 批准号: 7223351
• 负责人: RICHARD M. LEVENSON
• 金额: $ 19万
• 依托单位: CAMBRIDGE RESEARCH AND INSTRUMENTATION
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-05-01 至 2008-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7223351
• 关键词: 3-Dimensional; Acceleration; Address; Algorithms; American; Anesthesia procedures; Animal Disease Models; Animal Model; Animals; Atlases; Autopsy; Biological Models; Calibration; California; Cardiac; Cells; Chemistry; Cherry - dietary; Clinical Laboratory Information Systems; Collaborations; Collection; Communities; Compatible; Computer software; Containment; Custom; Data; Depth; Detection; Development; Disease; Disease model; Dyes; Electronics; Elements; Employment; Ensure; European; Evaluation; Eye; Face; Facility Construction Funding Category; Fluorescence; Focus Groups; Freezing; Frozen Sections; Generations; Glass; Goals; Government; Heating; Height; Hour; Image; Image Analysis; Imagery; Indium; Institutes; Joints; Label; Legal patent; Libraries; Light; Lighting; Liquid substance; Location; Lung; Maps; Measures; Mechanics; Methods; Modality; Modeling; Molecular Probes; Movement; Mus; Noise; Numbers; Operative Surgical Procedures; Optical Tomography; Optics; Other Imaging Modalities; Pattern; Performance; Pharmacologic Substance; Phase; Physiology; Play; Pliability; Principal Investigator; Probability; Process; Property; Publications; Purpose; Quantum Dots; Range; Reagent; Regulation; Reporter; Reporting; Research; Resolution; Risk; Role; Safety; Sampling; Shapes; Side; Signal Transduction; Site; Small Animal Imaging Systems; Small Business Funding Mechanisms; Small Business Innovation Research Grant; Software Tools; Software Validation; Son; Source; Speed; Standards of Weights and Measures; Structure; Submersion; Surface; System; Techniques; Testing; Time; Tissue Model; Tissues; Training; United States Food and Drug Administration; Universities; Validation; Variant; Vascular Diseases; Washington; Work; aged; base; design; digital; drug discovery; experience; fluorophore; improved; in vivo; indexing; instrument; interest; lens; light emission; lung imaging; malignant breast neoplasm; millimeter; mouse model; novel; optical imaging; performance tests; programs; prototype; reconstruction; research study; sensor; simulation; tool

DESCRIPTION (provided by applicant): This is a Fast-Track proposal to develop a multispectral optical tomography small-animal imaging system for use by the biomedical and pharmaceutical research communities. It will combine the experience CRI has acquired in multispectral, planar small-animal imaging (through its development of the Maestro(tm) system) with novel hyperspectral optical tomography algorithms and will be realized using a unique optical design. The primary aim of this proposal is to refine 3-dimensional (3D) localization of point and extended fluorescent sources within small animals. Tomographic approaches are essential for achieving truly quantitative, depth- resolved imaging. Partners in this project include leaders in optical design, algorithmic development, dye chemistry, advanced small-animal models of disease, and directors of animal imaging facilities. Key properties include: 1) the use of a spectrally tunable filter with unmatched flexibility for multiplexed fluorophore signal detection in the presence of significant autofluorescence; 2) a novel optic that enables multi- view detection; 3) a customized multispectral fluorescent label strategy; and 4) no requirement for camera or subject movement, compression of the subject between glass plates, or submersion of the subject in index- and scatter-matching fluids. Phase I will accomplish: construction of a first-generation instrument with off-the-shelf optics; radiometric characterization; surface profilometry, and confirmation via simulation that the instrument will accomplish 3D reconstructions with a resolution of 2 millimeters or better. Phase II will be dedicated to building a second- generation version of the instrument with custom, high-efficiency optics, and will add a variety of important algorithmic, computational and reagent components for optimal performance, with an emphasis on quantitative validation. The system will be deployed at several animal imaging facilities. A variety of animal models of disease, including metastatic breast cancer, pulmonary, cardiac and vascular disease will be studied and the performance of the Maestro-3D compared to competing optical and non-optical imaging modalities. In addition, CRI will work on methods to accelerate time consuming tomographic reconstructions. Finally, easy-to-use software for control, display, quantitation and integration into laboratory information systems will be developed.

描述（由申请人提供）：这是一项快速提案，旨在开发供生物医学和药物研究界使用的多光谱光学断层扫描小动物成像系统。它将把 CRI 在多光谱、平面小动物成像（通过开发 Maestro(tm) 系统）方面获得的经验与新颖的高光谱光学断层扫描算法相结合，并将使用独特的光学设计来实现。该提案的主要目的是完善小动物内点和扩展荧光源的 3 维 (3D) 定位。断层扫描方法对于实现真正定量、深度分辨成像至关重要。该项目的合作伙伴包括光学设计、算法开发、染料化学、先进小动物疾病模型的领导者以及动物成像设施的负责人。主要特性包括：1) 使用具有无与伦比灵活性的光谱可调滤光片，在存在显着自发荧光的情况下进行多重荧光信号检测； 2) 一种能够实现多视角检测的新型光学器件； 3）定制的多光谱荧光标记策略； 4) 不需要相机或物体移动、玻璃板之间压缩物体或将物体浸没在折射率和散射匹配液体中。第一阶段将完成： 建造具有现成光学器件的第一代仪器；辐射特性；表面轮廓测量，并通过模拟确认仪器将以 2 毫米或更好的分辨率完成 3D 重建。第二阶段将致力于构建具有定制高效光学器件的第二代仪器，并将添加各种重要的算法、计算和试剂组件以实现最佳性能，重点是定量验证。该系统将部署在多个动物成像设施中。将研究多种疾病动物模型，包括转移性乳腺癌、肺部、心脏和血管疾病，并将 Maestro-3D 的性能与竞争性光学和非光学成像方式进行比较。此外，CRI 将致力于研究加速耗时的断层扫描重建的方法。最后，将开发用于控制、显示、定量和集成到实验室信息系统的易于使用的软件。