Cancer Detection Using Diffuse Luminescence Imaging

描述（由申请人提供）：该提案的重点是使用氧敏磷光标记和混合成像模式进行乳腺癌成像和肿瘤表征。我们建议使用同时扩散光学断层扫描（DOT）和磁共振成像（MRI），以获得高空间分辨率和定量功能图像的乳腺肿瘤。由于许多临床和科学原因，使用注射的造影剂在临床前模型中成像以及对组织内的氧浓度成像是重要的。肿瘤中的低氧含量是放射抵抗性肿瘤区域的标志，并且也与不良治疗结果相关。对于基础研究应用，了解缺氧在肿瘤中如何发展以及它如何与低氧的外源性和内源性标志物相关也非常重要。在第一阶段，结合标准的扩散光学断层扫描（DOT），我们获得了高对比度图像的缺氧区域的乳腺组织模拟幻影使用长寿命的氧敏感造影剂和一个简单的寿命成像系统与冷却雪崩光电二极管。在第二阶段，将开发CW和时域寿命成像模块的原型，该模块将在冷却的16元件雪崩光电二极管（APD）阵列上进行并行采集，以实现高分辨率和高通量成像。低成本、紧凑型使用寿命成像仪模块将被设计为与混合成像系统中与MR单元相关的高磁场和RF场兼容。在我们的合作者位于新罕布什尔州达特茅斯学院的机构（Brian Pogue教授，Thayer工程学院）的双模态成像装置中使用该APD模块，将进行体模和动物模型成像，以获得高分辨率和高对比度的功能光学图像。光学图像重建将由从同时获得的高分辨率MR图像获得的空间和解剖信息辅助，以提高这些图像的准确性。基于紧凑型APD的探测器原型将具有较小的占地面积，并将安装在MRI单元的磁体内部，从而显著降低当前基于PMT的探测系统的复杂性。公共卫生相关性：具有同时扩散发光成像和磁共振成像的混合成像可用于基于肿瘤的氧浓度获得肿瘤的高分辨率功能图。低渗乳腺癌检测是主要目标应用，目的是使用外源性造影剂提高诊断准确性和治疗预后。其他应用可以包括监测脑功能或脑出血的脑成像，以及监测组织和肌肉氧合、组织毒性等。

DESCRIPTION (provided by applicant): The focus of the proposal is in the area of breast cancer imaging and tumor characterization using oxygen sensitive phosphorescent labels and hybrid imaging modality. We propose to use simultaneous diffuse optical tomography (DOT) and magnetic resonance imaging (MRI) to obtain high spatial-resolution and quantitative functional images of breast tumors. Imaging in pre-clinical models with injected contrast agents and imaging the oxygen concentration within tissue are important for a number of clinical and scientific reasons. Low oxygen content in tumors is a marker of radiation-resistant tumor regions, and is also correlated with a poor treatment outcome. For basic research applications, it is also very important to understand how hypoxia develops in tumors and how it is correlated with exogenous and endogenous markers of low oxygen. During Phase I, in conjunction with standard diffuse optical tomography (DOT), we obtained high contrast images of hypoxic regions in breast tissue simulating phantoms using long-lived oxygen sensitive contrast agents and a simple lifetime imaging system with cooled avalanche photodiodes. In Phase II, a prototype of the CW and time domain lifetime imaging module will be developed which will have parallel acquisition across a cooled 16-element avalanche photodiode (APD) array for high resolution and high throughput imaging. The low-cost, compact lifetime imager module will be designed to be compatible with high magnetic and RF fields associated with MR units in hybrid imaging systems. Using this APD module in a dual-modality imaging setup at our collaborator's facility at Dartmouth College, NH (Prof. Brian Pogue, Thayer School of Engineering), phantom and animal model imaging will be performed to obtain functional optical images with high resolution and high contrast. The optical image reconstruction will be aided by the spatial and anatomical information obtained from simultaneously obtained high-resolution MR images in order to improve the accuracy of these images. The compact APD based detector prototype will have a small footprint and will be mounted inside the magnet of the MRI unit, dramatically decreasing the complexity of the current PMT based detection systems. PUBLIC HEALTH RELEVANCE: Hybrid imaging with simultaneous diffuse luminescence imaging and magnetic resonance imaging can be used to obtain high resolution functional maps of tumors based on their oxygen concentration. Hypoxic breast cancer detection is the primary target application, with the goal of improving diagnostic accuracy and treatment prognosis using exogenous contrast agents. Other applications could include brain imaging to monitor cerebral function or cerebral hemorrhage, and monitoring tissue and muscle oxygenation, histotoxicity, etc.