XAS CHARACTERIZATION OF FE SITES IN PROTEINS INVOLVED IN IRON HOMEOSTASIS & CELL

参与铁稳态的蛋白质中 FE 位点的 XAS 表征

• 批准号: 7597947
• 负责人: TIMOTHY Louis STEMMLER
• 金额: $ 0.4万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-03-01 至 2008-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7597947
• 关键词: Active Sites; Anabolism; Cell Respiration; Cell Survival; Cell physiology; Cells; Computer Retrieval of Information on Scientific Projects Database; Funding; Goals; Grant; Heme Iron; Homeostasis; Institution; Iron; Metalloproteins; Metals; Mitochondria; Oxidative Stress; Proteins; Reaction; Research; Research Personnel; Resources; Role; Site; Source; Spectrum Analysis; Structure; Sulfur; United States National Institutes of Health; cofactor; enzyme pathway; metal poisoning; prevent

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Although mitochondrial iron homeostasis is essential for cell viability, it is a mechanism that is poorly understood. Mitochondrial iron is required for heme and iron-sulfur cluster assembly and deficiency causes a collapse in metalloprotein biosynthesis, disrupting cellular pathways where enzymes that require these cofactors participate. However, overabundance of mitochondrial iron leads to oxidative stress in the cell, since iron is highly reactive. Cells have therefore developed protein controlled mechanisms to regulate mitochondrial iron concentrations at the level of metal import, export and possibly metal storage. Cellular respiration is controlled in the mitochondria by metalloproteins, so strict control of mitochondrial iron concentrations is essential for maintaining cell function and also to prevent metal toxicity. A goal of our lab is to characterize the function of two proteins involved in mitochondrial iron homeostasis and cellular respiration. Using XAS spectroscopy, we will characterize the iron active-site structure and reaction mechanism for these proteins to decipher their role in maintaining normal cellular function.

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 尽管线粒体铁稳态对细胞存活至关重要，但人们对其机制知之甚少。线粒体铁是血红素和铁-硫簇组装所必需的，缺乏会导致金属蛋白生物合成的崩溃，扰乱需要这些辅因子的酶参与的细胞途径。然而，线粒体铁的过量会导致细胞中的氧化应激，因为铁是高度活跃的。因此，细胞已经发展出蛋白质控制的机制，在金属进出口和可能的金属储存水平上调节线粒体铁的浓度。线粒体中的呼吸是由金属蛋白控制的，因此严格控制线粒体中铁的浓度对于维持细胞功能和防止金属中毒是必不可少的。我们实验室的一个目标是确定参与线粒体铁稳态和细胞呼吸的两种蛋白质的功能。利用XAS光谱，我们将表征这些蛋白质的铁活性部位结构和反应机制，以破译它们在维持正常细胞功能方面的作用。