COLLECTION OF PROTEIN CRYSTALLOGRAPHIC DATA

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。列出的机构是 研究中心，而研究中心不一定是研究者所在的机构。 Syrrx是一家位于圣地亚哥的结构蛋白质组学公司，从事基于结构的药物设计。 几个目标的微生物蛋白质已结晶在Syrrx，其特征在于与单色X射线。 为了定相这些，我们已经用Se-Met取代生长和结晶这些蛋白质，并且正在寻求MAD束时间。 我们将在光束线9-2收集蛋白质晶体X射线衍射数据。我们将使用标准的光束线操作程序和设备。所有晶体都被带到SSRL进行预冷冻，并将在实验结束时从现场移除。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Syrrx is a structural proteomics company based in San Diego doing structure-based drug design. Several targets of microbial proteins have been crystallized at Syrrx, characterized with monochromatic X-rays. In order to phase these we have grown and crystallized these proteins with Se-Met substitution and are seeking MAD beam time. We will be collecting protein crystal X-ray diffraction data at beamline 9-2. We will be using standard beamline operating procedures and equipment. All crystals are being brought to SSRL pre-frozen and will be removed from the site at the end of the experiment.