COLLECTION OF PROTEIN CRYSTALLOGRAPHIC DATA

本子项目是利用由NIH/NCRR资助的中心赠款提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是中心的，不一定是研究者的机构。Syrrx是一家位于圣地亚哥的结构蛋白质组学公司，从事基于结构的药物设计。微生物蛋白的几个靶标已经在Syrrx上结晶，用单色x射线表征。为了使这些蛋白质相化，我们用Se-Met取代生长和结晶这些蛋白质，并寻求MAD束时间。我们将在光束线9-2处收集蛋白质晶体x射线衍射数据。我们将使用标准的光束线操作程序和设备。所有的晶体都被带到SSRL进行预冻，并将在实验结束时从现场移走

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Syrrx is a structural proteomics company based in San Diego doing structure-based drug design. Several targets of microbial proteins have been crystallized at Syrrx, characterized with monochromatic X-rays. In order to phase these we have grown and crystallized these proteins with Se-Met substitution and are seeking MAD beam time. We will be collecting protein crystal X-ray diffraction data at beamline 9-2. We will be using standard beamline operating procedures and equipment. All crystals are being brought to SSRL pre-frozen and will be removed from the site at the end of the experiment