USER TRAINING AND SUPPORT FOR BIO-SAXS/D DATA ACQUISITION AND DATA ANALYSIS SOFT

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 SSRL BTP工作人员为有效使用SMB SAXS/D仪器提供实践用户培训。用户会收到关于该软件的简短教程，供他们选择数据采集系统。所有用户现在都使用BLU-ICE进行非时间分辨的数据收集，用于溶液散射研究和部分定向系统的研究。他们接受关于MarParse的培训，MarParse是BTP工作人员最近开发的溶液散射数据处理程序。指导用户了解溶液x射线散射数据分析的基础知识，例如检查数据中可能出现的辐射引起的聚集、减去背景和计算旋转半径。欧洲同步辐射设施开发的数据处理程序Fit2D以及EMBL-Hamburg的Primus也应要求提供。还给出了由Svergan博士(EMBL-Hamburg)开发的复杂解散射数据解释软件包的一些入门教程。对实验几何和设备的选择，特别是探测器的选择和数据采集策略提出了实用的建议，特别是为了最大限度地减少辐射损伤，以帮助提高数据质量。这是对新用户组的常规操作，已被发现对有经验的用户也很有用。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The SSRL BTP staff provides hands-on user training for the effective use of the SMB SAXS/D instrument. Users receive a short tutorial on the software for their choice of data collection system. All users now use Blu-ICE for non-time resolved data collection for both solution scattering studies and studies on partially oriented systems. They receive training on MarParse, the data processing program for solution scattering developed recently by the BTP staff. Users are instructed on the basics in solution x-ray scattering data analysis, such as inspecting data for possible radiation-induced aggregation, subtracting the background, and calculating radii of gyration. The data processing program Fit2D, developed at the European Synchrotron Radiation Facility, as well as Primus from EMBL-Hamburg are also covered upon request. Some introductory tutorials to the sophisticated solution scattering data interpretation software packages, developed by Dr. Svergun (EMBL-Hamburg) are also given. Practical advice is given on choice of experiment geometry and equipment, especially detector, and data collection strategy especially for minimizing radiation damage to help improve data quality. This is routinely done to new user groups and has been found useful also for experienced users.