USER TRAINING AND SUPPORT FOR BIO-SAXS/D DATA ACQUISITION AND DATA ANALYSIS SOFT

该子项目是利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得主要资金， 因此可以在其他CRISP条目中表示。列出的机构是 中心，不一定是研究者的机构。 SSRL BTP工作人员为有效使用SMB SAXS/D仪器提供实际操作用户培训。 用户收到一个简短的教程软件为他们选择的数据收集系统。所有用户现在都使用Blu-ICE进行溶液散射研究和部分定向系统研究的非时间分辨数据收集。 他们接受了MarParse的培训，这是BTP员工最近开发的用于解决方案分散的数据处理程序。 用户被指示在解决方案的X射线散射数据分析的基础知识，如检查数据可能的辐射诱导的聚集，减去背景，并计算回转半径。 数据处理程序Fit 2D，在欧洲同步辐射设施，以及从EMBL-Hamburg的Primus开发的也包括在要求。 Svergun博士（EMBL-汉堡）开发的复杂的解决方案散射数据解释软件包的一些介绍性教程也给出了。 对实验几何结构和设备的选择，特别是探测器，以及数据收集策略，特别是为了最大限度地减少辐射损伤，以帮助提高数据质量，给出了实用的建议。 这是对新用户组的常规操作，对有经验的用户也很有用。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The SSRL BTP staff provides hands-on user training for the effective use of the SMB SAXS/D instrument. Users receive a short tutorial on the software for their choice of data collection system. All users now use Blu-ICE for non-time resolved data collection for both solution scattering studies and studies on partially oriented systems. They receive training on MarParse, the data processing program for solution scattering developed recently by the BTP staff. Users are instructed on the basics in solution x-ray scattering data analysis, such as inspecting data for possible radiation-induced aggregation, subtracting the background, and calculating radii of gyration. The data processing program Fit2D, developed at the European Synchrotron Radiation Facility, as well as Primus from EMBL-Hamburg are also covered upon request. Some introductory tutorials to the sophisticated solution scattering data interpretation software packages, developed by Dr. Svergun (EMBL-Hamburg) are also given. Practical advice is given on choice of experiment geometry and equipment, especially detector, and data collection strategy especially for minimizing radiation damage to help improve data quality. This is routinely done to new user groups and has been found useful also for experienced users.