LASER-CROSSLINKING OF MICRO-RNPS ON THEIR MRNA TARGET

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 microRNA（miRNAs）与属于Argonaute家族（Ago）的蛋白质结合。miRNA通过与其mRNA靶标中发现的miRNA识别元件（MRE）的碱基配对来发挥功能。我们先前将位点特异性可光交联的核苷酸掺入MRE中，使得UV照射在MRE与其结合蛋白之间形成共价键。我们想调查使用的第四谐波的Nd：YAG激光器的这种光交联。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. MicroRNAs (miRNAs) are bound to proteins that belong to the Argonaute family (Ago). miRNAs function by base pairing with miRNA-recognition elements (MREs) found in their mRNA targets. We previously incorporated site-specific photocrosslinkable nucleotides nto an MRE so that UV irradiation forms covalent bonds between the MRE and its binding protein. We would like to investigate the use of the fourth harmonic of a Nd:YAG laser for this photocrosslinking.