MEASUREMENT OF SITE SPECIFIC PROTON, NITROGEN, AND CARBONYL CHEMICAL SHIELDING

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This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The changes in a solute's chemical shifts between an isotropic and a liquid crystalline phase provide information on the magnitude and orientation of the chemical shielding anisotropy (CSA) tensors relative to the molecule's alignment frame. Such chemical shift changes will be measured for the polypeptide backbone C', N, and HN resonances in the protein GB3, dissolved in at least 5 different alignment media needed to calculate the site specific CSA values (as functions of 5 independent variables). The one-bond 1H-15N dipolar couplings provide a reference to determine the protein alignment tensor.