REGULATION OF LUNG DEVELOPMENT AND DISEASE

肺部发育和疾病的调节

• 批准号: 7601211
• 负责人: Mary E. Sunday
• 金额: $ 0.5万
• 依托单位: DUKE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-07-01 至 2008-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7601211
• 关键词: Age; Aging; Animals; Birth; Computer Retrieval of Information on Scientific Projects Database; Disease; Drosophila genus; Funding; Genes; Grant; Heterozygote; Homologous Gene; Hour; Individual; Institution; Knockout Mice; Life; Lung; Mus; Phenotype; Pulmonary Emphysema; Regulation; Research; Research Personnel; Resources; Respiratory Failure; Respiratory System; Source; Structure of parenchyma of lung; Time; United States National Institutes of Health; X-Ray Computed Tomography; lung development; mutant; research study

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We cloned npas3 as the mouse homolog of a Drosophila gene, trachealess, the null mutant of which completely lacks a respiratory system. 90% of our npas3-null mice die within a few hours after birth of respiratory failure, with severely emphysematous lungs. The npas3 heterozygotes (Het) live, but have varying degrees of emphysema and/or reactive airways disease. We don't know whether there are differences in the lung phenotype at different ages (i.e. worsening of emphysema with aging) or whether there is individual variability between different animals. Micro-CT scans will permit analysis of the lung tissue of individual animals at different time points. Comparison of one npas3-Het and one wild-type littermate is an important pilot experiment for the competing renewal of our grant.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 我们将 npas3 克隆为果蝇基因的小鼠同源物，无气管，其无效突变体完全缺乏呼吸系统。 90% 的 npas3 缺失小鼠在出生后数小时内死于呼吸衰竭，并伴有严重的肺部气肿。 npas3 杂合子 (Het) 存活，但患有不同程度的肺气肿和/或反应性气道疾病。我们不知道不同年龄的肺表型是否存在差异（即肺气肿随着年龄的增长而恶化），或者不同动物之间是否存在个体差异。微型 CT 扫描将允许在不同时间点分析个体动物的肺组织。比较一个 npas3-Het 和一个野生型同窝动物是我们竞争性更新资助的一个重要试点实验。