CIMA (CASPASE-INDEPENDENT MITOTIC APOPTOSIS)

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 为了帮助研究线粒体的结构，对线粒体进行电子显微镜和电子断层扫描的珀金斯博士同意观察我们处理过的细胞。纺锤体检查点与细胞死亡或凋亡之间的关系知之甚少。许多肿瘤细胞的纺锤体检查点反应减弱，但不是缺失。当Bub1功能部分但不像染色体不稳定(CIN)肿瘤那样完全中断时，就会发生CIMCD。CIMCD可能是一种防止非整倍体的替代系统，因此，当Bub1介导的纺锤体检查点激活失败时，可能会发生肿瘤。珀金斯博士正在提供培养细胞中CIMCD的结构分析，不仅是线粒体的结构分析，还包括一般细胞质特征的结构分析。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. In order to aid the study of mitochondrial structure, Dr. Perkins, who conducts electron microscopy and electron tomography on mitochondria, agreed to look at our treated cells. Little is known about the relationship between the spindle checkpoint and cell death or apoptosis. Many tumor cells have a diminished, but not absent, spindle checkpoint response. caspase-independent mitotic cell death (CIMCD) occurs when BUB1 function is partly but not completely disrupted as in chromosome instability (CIN) tumors. CIMCD may be an alternative system to prevent aneuploidy and, therefore, probably tumorigenesis when the BUB1-mediated activation of the spindle checkpoint fails. Dr. Perkins is providing a structural analysis of CIMCD in cultured cells, not only of mitochondria, but also of general cytoplasmic features.