ENVIRO SENSING, METABOLIC RESPONSE, & REGULATORY NETWORKS IN RESPIRATORY SYS

该子项目是利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得主要资金， 因此可以在其他CRISP条目中表示。列出的机构是 中心，不一定是研究者的机构。 希瓦氏菌MR-1是一种能动的兼性细菌，在电子受体利用方面具有显著的代谢多样性;它可以利用O2、硝酸盐、富马酸盐、Mn、Fe和SO作为呼吸过程中的末端电子受体。这种多功能性使MR-1能够在电子受体类型和浓度在空间和时间上波动的环境中有效地竞争资源。有效地减少多价金属和放射性核素，包括固相铁和锰氧化物的能力，产生了相当大的兴趣，这种生物体的潜在作用，在地球化学循环和污染金属和放射性核素的生物修复。尽管付出了相当大的努力，MR-1的电子传输系统的细节以及它还原金属和放射性核素的机制仍然不清楚。关于这种生物体中的分子网络，使其能够在不断变化的环境中有效地竞争，我们所知甚少。MR-1的整个基因组序列基本上已经确定，现在可以使用高通量方法测量基因表达，包括PNNL开发的基于质谱的蛋白质组分析。虽然功能强大，但DNA阵列和蛋白质组分析必须与其他方法紧密结合，以有效揭示MR-1如何在其环境中发挥作用并对其做出反应的分子细节。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Shewanella confidences MR-1 is a motile facultative bacterium with remarkable metabolic versatility in regards to electron acceptor utilization; it can utilize O2, nitrate, fumarate, Mn, Fe, and S0 as terminal electron acceptors during respiration. This versatility allows MR-1 to efficiently compete for resources in environments where electron acceptor type and concentration fluctuate in space and time. The ability to effectively reduce polyvalent metals and radionuclides, including solid phase Fe and Mn oxides, has generated considerable interest in the potential role of this organism in biogeochemical cycling and in the bioremediation of contaminant metals and radionuclides. In spite of considerable effort, the details of MR-1's electron transport system and the mechanisms by which it reduces metals and radionuclides remain unclear. Even less is known regarding the molecular networks in this organism that allow it to respond to compete efficiently in a changing environment. The entire genome sequence of MR-1 has, in essence, been determined and high throughput methods for measuring gene expression are now available, including mass spec-based proteome analyses developed at PNNL. Although powerful, DNA array and proteome analyses must be tightly coupled with other approaches to effectively reveal the molecular details of how MR-1 functions in, and responds to, its environment.