ENVIRONMENT SENSING, METAB RESPONSE & REGULATORY NETWORK IN RESPIRATORY PATHWAY

描述：国家显微镜与成像研究中心（NCMIR）的建立是为了开发计算机辅助高级显微镜，用于获取尺寸范围为1nm3至100um3的结构和功能数据。借助新的标本染色方法、成像仪器和计算能力，研究人员正在解决后基因组时代的下一个重大生物学挑战，即在细胞和组织环境中定位蛋白质和大分子复合物。资源仪器包括中高压透射电子显微镜（IVEMs）和高速大幅面激光扫描光显微镜，专门用于“中尺度”生物显微镜。我们的核心技术研究方向是开发有助于结构神经生物学，细胞和分子生物学的技术。随着NCMIR在生物医学信息学研究网络（BIRN）中的参与，我们正在扩大我们在大范围脑组织中进行高分辨率成像的能力，以揭示小鼠的结构和分子

DESCRIPTION: The National Center for Microscopy and Imaging Research (NCMIR) was established to develop computer aided advanced microscopy for acqusition of structural and functional data in the dimensional range of 1 nm3 to 100 um3. With novel specimen staining methods, imaging instruments and computational capabilities, researchers are addressing the next great biological challenges in the post-genomic age by situating proteins and macromolecular complexes in their cellular and tissue environements. Resource instruments include intermediate high voltage transmission electron microscopes (IVEMs) and high-speed large-format laser-scanning light microscopes specially modified for "meso-scale" biological microscopy. Our core technological research is directed towards developing technologies that will contribute to structural neurobiology, cell and molecular biology. With NCMIR's participation in the Biomedical Informatics Research Network (BIRN), we are scaling up our ability to image at high resolution across wide expanses of brain tissue to reveal structural and molecular in mouse models of human disease. Our collaboration, service, training and dissemination programs expand the use of these technologies to maximize their value to the biomedical community. Core biological projects include development of new staining methods for IVEM and for correlated light and IVEM analyses of specimens to examine the architecture of neuronal systems and the dynamics of subcellular processes. Core research is tightly linked to collaborative projects, in areas such as animal of neurodegenerative diseases, stroke, heart disease, cancer, infectious diseases and diabetes. The capabilites and research accomplishments anticipated in specimen preparation are augmented by continued development of instruments and image processing facilities. We emphasize the use of computer-aided methods for enhancing image contrast and reconstructing larger structural complexes using 3D tomographic and serial thick section analysis. Extraction of 3D information on a large scale will be accelerated through the development of high-throughput methods for tomographic reconstruction, analysis and visualization. We continue to provide researchers greater access to the Resource through development of web-based remote control systems for the IVEM's and interfaces for computational grids and distributed databases via high-speed networks. We will expand outreach efforts via web-based tutorials, software and microscopic data repositories linked to other biological databases.