ENVIRO SENSING, METABOLIC RESPONSE, & REGULATORY NETWORKS IN RESPIRATORY SYS

环境传感、代谢反应、

• 批准号: 7722329
• 负责人: JAMES FREDRICKSON
• 金额: $ 0.32万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-05-01 至 2009-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7722329
• 关键词: Bacteria; Bioremediations; Computer Retrieval of Information on Scientific Projects Database; Coupled; DNA; Electron Transport; Environment; Fumarates; Funding; Gene Expression; Grant; Institution; Measures; Metabolic; Metals; Methods; Molecular; Nitrates; Organism; Oxidants; Oxides; Phase; Proteome; Radioisotopes; Research; Research Personnel; Resources; Respiration; Role; Shewanella; Solid; Source; System; Time; United States National Institutes of Health; base; genome sequencing; interest; nitrate; respiratory; response

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Shewanella confidences MR-1 is a motile facultative bacterium with remarkable metabolic versatility in regards to electron acceptor utilization; it can utilize O2, nitrate, fumarate, Mn, Fe, and S0 as terminal electron acceptors during respiration. This versatility allows MR-1 to efficiently compete for resources in environments where electron acceptor type and concentration fluctuate in space and time. The ability to effectively reduce polyvalent metals and radionuclides, including solid phase Fe and Mn oxides, has generated considerable interest in the potential role of this organism in biogeochemical cycling and in the bioremediation of contaminant metals and radionuclides. In spite of considerable effort, the details of MR-1's electron transport system and the mechanisms by which it reduces metals and radionuclides remain unclear. Even less is known regarding the molecular networks in this organism that allow it to respond to compete efficiently in a changing environment. The entire genome sequence of MR-1 has, in essence, been determined and high throughput methods for measuring gene expression are now available, including mass spec-based proteome analyses developed at PNNL. Although powerful, DNA array and proteome analyses must be tightly coupled with other approaches to effectively reveal the molecular details of how MR-1 functions in, and responds to, its environment.

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 自信希瓦氏杆菌MR-1是一种运动性兼性细菌，在电子受体利用方面具有显著的代谢多样性；它能在呼吸过程中利用O2、硝酸盐、富马酸、锰、铁和S0作为末端电子受体。这种多功能性使MR-1能够在电子受体类型和浓度在空间和时间上波动的环境中有效地竞争资源。能够有效地还原多价金属和放射性核素，包括固相铁和锰氧化物，引起了人们对这种生物在生物地球化学循环和污染金属和放射性核素的生物修复中的潜在作用的极大兴趣。尽管做出了相当大的努力，但MR-1的S电子传输系统的细节以及它还原金属和放射性核素的机制仍不清楚。关于这种有机体中的分子网络，使其能够在不断变化的环境中做出反应并有效竞争，人们所知的更少。从本质上讲，MR-1的整个基因组序列已经确定，现在已经有了测量基因表达的高通量方法，包括PNNL开发的基于质谱学的蛋白质组分析。尽管功能强大，DNA阵列和蛋白质组分析必须与其他方法紧密结合，才能有效地揭示MR-1如何在其环境中发挥作用和对其做出反应的分子细节。