FIBER DIFFRACTION FROM FILAMENTOUS PLANT VIRUSES

丝状植物病毒的纤维衍射

• 批准号: 7601739
• 负责人: GERALD J STUBBS
• 金额: $ 2.06万
• 依托单位: ILLINOIS INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-04-01 至 2008-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7601739
• 关键词: Biotechnology; Computer Retrieval of Information on Scientific Projects Database; Data; Development; Family; Fiber; Filament; Funding; Glycine max; Grant; Humidity; Institution; Methods; Modeling; Mosaic Viruses; Necrosis; Pattern; Plant Viruses; Pliability; Potato Virus X; Potyvirus; Preparation; Research; Research Personnel; Resolution; Resources; Sampling; Source; Soybeans; Specimen; Structure; Tobacco Mosaic Virus; United States National Institutes of Health; Viral; Virus; bean; improved; member; method development; three dimensional structure

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We are collecting fiber diffraction data from flexible filamentous plant viruses, invaluable as models for recognition, assembly, flexibility, viral-host interactions, and in methods development. Plant viruses have great potential in biotechnology, but development will require three-dimensional structures. Fiber diffraction is often the only method available for the determination of such filamentous structures. Diffraction samples are oriented sols and dried fibers. We find that order is better in dried fibers of highly flexible filaments, and in oriented sols of more rigid filaments. Dried fibers require much less material, an important consideration for studies of low-titer viruses. Wide and small angle diffraction data are collected at BioCAT. We have obtained fiber diffraction data from bean common mosaic virus and bean common mosaic necrosis virus, significantly improved both in orientation and in resolution by the use of high humidities in specimen preparation. We have obtained even better patterns from two strains of the related potyvirus soybean mosaic virus (SMV); these are the first diffraction data obtained from SMV, and the best diffraction data ever obtained from a member of the important Potyvirus family. They will be invaluable in determining high-resolution potyvirus structures. We have obtained excellent diffraction patterns from oriented sols of tobacco mosaic virus and potato virus X, made using a new method that requires less than 5% of the material needed for conventional methods, and involves much less handling . The method will be extremely useful in fiber diffraction studies when samples are limited in quantity, labile, or hazardous.

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