SEARCHING FOR PROTEIN INTERACTING WITH YEAST MPH1

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 Mph1是酵母Fanconi贫血M(FANCM)的同源物，是3‘-5’DNA解旋酶。已有研究表明，DNA损伤的避免是通过与同源重组途径的遗传相互作用来实现的。然而，它与其他已知的DNA代谢蛋白的相互作用尚不清楚。为了了解Mph1是否与任何DNA代谢蛋白相互作用，我建议在没有或存在DNA损伤剂MMS的情况下筛选酵母双杂交文库。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Mph1, a yeast Fanconi Anemia M (FANCM) homolog is 3'-5' DNA helicase. It has been suggested to function in DNA damage avoidance through genetic interaction with homologous recombination pathway. However, its interaction with other known DNA metabolism proteins is not clearly understood. To understand whether Mph1 interacts with any DNA metabolism proteins, I propose to screen yeast two hybrid library in the absence or presence of DNA damaging agent, MMS.