SEARCHING FOR PROTEIN INTERACTING WITH YEAST MPH1

这个子项目是众多研究子项目之一

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Mph1, a yeast Fanconi Anemia M (FANCM) homolog is 3'-5' DNA helicase. It has been suggested to function in DNA damage avoidance through genetic interaction with homologous recombination pathway. However, its interaction with other known DNA metabolism proteins is not clearly understood. To understand whether Mph1 interacts with any DNA metabolism proteins, I propose to screen yeast two hybrid library in the absence or presence of DNA damaging agent, MMS.