SEARCHING FOR PROTEIN INTERACTING WITH YEAST MPH1

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 Mph 1是一种酵母Fanconi贫血M（FANCM）同系物，是3 '-5' DNA解旋酶。已被认为是通过与同源重组途径的遗传相互作用在DNA损伤避免中起作用。然而，它与其他已知的DNA代谢蛋白的相互作用还不清楚。为了了解Mph 1是否与任何DNA代谢蛋白相互作用，我建议在不存在或存在DNA损伤剂MMS的情况下筛选酵母双杂交文库。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Mph1, a yeast Fanconi Anemia M (FANCM) homolog is 3'-5' DNA helicase. It has been suggested to function in DNA damage avoidance through genetic interaction with homologous recombination pathway. However, its interaction with other known DNA metabolism proteins is not clearly understood. To understand whether Mph1 interacts with any DNA metabolism proteins, I propose to screen yeast two hybrid library in the absence or presence of DNA damaging agent, MMS.