Genome Instability in Cancer Development

癌症发展中的基因组不稳定性

• 批准号: 8750677
• 负责人: Kyungjae Myung
• 金额: $ 75.26万
• 依托单位: NATIONAL HUMAN GENOME RESEARCH INSTITUTE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8750677
• 关键词: Affect; Aftercare; Aneuploidy; Aphidicolin; Ataxia Telangiectasia; BRCA1 Protein; BRCA2 gene; Biochemistry; Bypass; Cell Culture Techniques; Cell Death; Cell Nucleolus; Cell Proliferation; Cell Survival; Cells; Chromosomal Rearrangement; Chromosome Arm; Chromosomes; Collaborations; Complement; Complementary DNA; Complex; Congenital Abnormality; DNA Damage; DNA Repair; DNA Repair Pathway; DNA biosynthesis; DNA repair protein; DNA replication fork; Daphne plant; Development; Disease; Embryo; Endometrial Neoplasms; Fanconi anemia protein; Fanconi' s Anemia; Fungal Genome; Future; Gene Activation; Generations; Genes; Genetic; Genetic Polymorphism; Genetic Recombination; Genetic Transcription; Genome; Genomic Instability; Goals; Hereditary Disease; Histone H3; Histones; Homologous Gene; Homologous Protein; Human; Human Cloning; Human Genome; Incidence; Inherited; Journals; Knockout Mice; Lead; Libraries; Link; Malignant Neoplasms; Mammals; Mediating; Metabolism; Methods; Modeling; Modification; Molecular; Molecular Biology; Mus; Mutate; Mutation; National Human Genome Research Institute; Open Reading Frames; Orthologous Gene; Pancytopenia; Pathogenesis; Pathway interactions; Patients; Phenotype; Phosphorylation; Phosphotransferases; Play; Postdoctoral Fellow; Predisposition; Prevention strategy; Proliferating Cell Nuclear Antigen; Proteins; Proto-Oncogenes; RNA Polymerase I; Regulation; Regulation of Proteolysis; Reporting; Ribosomal RNA; Role; S Phase; SMARCA3 gene; Sequence Homology; Signal Transduction; Sister Chromatid; Somatic Mutation; Source; Stress; Syndrome; System; Testing; Tumor Suppressor Proteins; Ubiquitin; Ubiquitination; Work; Yeast Model System; Yeasts; Zebrafish; activator 1 protein; base; cancer cell; cancer therapy; genome database; genome wide association study; homologous recombination; human FRAP1 protein; human disease; hydroxyurea; interstitial; irradiation; malignant breast neoplasm; novel; null mutation; overexpression; prevent; protein complex; protein function; repaired; response; screening; small hairpin RNA; tumor; tumorigenesis; yeast genetics

Transmitting genetic information without creating deleterious genetic alterations is one of the most important tasks. Cells have evolved systems that check for and repair potentially lethal DNA damage. However, when these systems do not work properly, DNA damage accumulates and causes genetic changes or cell death. Accumulation of genetic changes, which is defined as a genomic instability is frequently observed in various types of genetic disorders including cancers. Genomic instability has been documented as a preceding step for multiple inactivations of tumor suppressor genes and activations of proto-oncogenes. One type of genomic instability observed frequently in many cancers is gross chromosomal rearrangement (GCR). GCR includes translocations, deletions of chromosome arm, interstitial deletions, inversions, amplifications, chromosome end-to-end fusion and aneuploidy. Although little is known about the origin and mechanisms of GCRs observed in cancer cells, recent studies on genes mutated in inherited cancer predisposition syndromes have started to demonstrate that proteins that function in DNA damage responses, DNA repair, and DNA recombination, play crucial roles in the suppression of spontaneous and/or DNA damage-induced GCRs. The recent identification of strong correlations between genes responsible for genetic diseases including cancers and GCRs started to pinpoint the importance of GCRs. To understand mechanisms how GCRs are generated and how such GCR formation can lead tumorigenesis, we screened the entire yeast genome for mutations or overexpression that increase the rate of GCR formation. RAD5 and ELG1 from mutation screening and MPH1 from overexpression screening were selected for further studies of molecular mechanisms of these proteins to protect genome from deleterious GCR formation. 1. Determine the role of RAD5 orthologs in mammalian GCR and further dissect the RAD5 pathway upstream signals and additional factors. Previously, we identified two RAD5 orthologs in mammals and demonstrated that RAD5 orthologs, SHPRH and HLTF function to prevent collapse of persistent stalled replication forks by assisting template switching DNA damage bypass mechanism that uses the nascent strand of the sister chromatid for recombination mechanism for damage bypass. Among different modifications of Proliferating Cell Nuclear Antigen (PCNA) that determine the bypass mechanisms, we demonstrated that PCNA is poly-ubiquitinated by SHPRH and HLTF. Last year, we hypothesized that mice deficient in SHPRH would show a high incidence of tumorigenesis. We found that shprh-/- and hltf-/- and double knockout mice did not observe high level of tumorigenesis. In collaboration with Dr. Heinz Jacobs, we found that there is a redundant pathway that can complement the lack of SHPRH/HLTF pathway. SHPRH has a unique histone interaction domain called PHD domain. We recently found that this domain is important for SHPRH localization in the nucleolus. The localized SHPRH in the nucleolus regulate the transcription of rRNA and assist cellular proliferation. We found that rRNA expression by SHPRH was mediated by specific histone H3 modification and recruitment of RNA polymerase I. However, such new role of SHPRH in rRNA transcription was not affected by its original function that we found in DNA damage response. Lastly, we found that rRNA transcription by SHPRH depended on mTOR pathway that is important for cellular metabolism. 2. ATAD5 (mammalian ELG1 homolog): determine whether alternative Replication Factor C (RFC) complex protein directs DNA repair pathways and replication. To investigate whether the role of ELG1 in GCR suppression in yeast is conserved in mammals, we cloned the human ELG1 gene (ATAD5) by conducting a sequence homology search in the human genome database. Previously, we demonstrated that the reduced expression of the ATAD5 gene by shRNA increased spontaneous DNA damage resulted as evidenced by an increase of phosphorylated histone H2Ax and ATM foci. The ATAD5 protein was localized at the stalled replication fork after hydroxyurea treatment. We also demonstrated an increase of human ATAD5 expression at S-phase and after treatment of cells with various DNA-damaging agents, including MMS, hydroxyurea, aphidicolin, and gamma-irradiation. Previously, we demonstrated that ATAD5 interacts with PCNA and USP1 that removes ubiquitin from PCNA after DNA damage bypass pathway. In addition, we reported that mice haploinsufficient in Atad5 showed a high incidence of tumorigenesis. We recently confirmed that embryonic day 7.5 to 8.5 as embryonic lethality caused by homozygous null mutation of ATAD5. In addition, in collaboration with Dr. Daphne Bells group in NHGRI, we found human somatic mutations of ELG1 gene in many endometrial tumors. We also found several rare polymorphisms as well as cancer mutations in other tumor types. We are currently testing whether these rare mutations found in affect ATAD5s molecular function and cause phenotypes observed in mice and zebrafish. Lastly, in addition to its role in DNA repair, we found that ATAD5 functions in DNA replication through its interaction with PCNA. ATAD5 unloads PCNA when DNA replication ends at the end of S phase and at each cycle of lagging strand synthesis. 3. Determine the role of Mph1, the yeast homolog of FANCM, in DNA repair By screening genes that enhance GCR formation when overexpressed, we identified MPH1 as the strongest GCR enhancing gene. MPH1 has been implicated in a homologous recombination-dependent DNA repair pathway. Recently, the human homolog of MPH1 was discovered as the gene mutated in FA complementation group M (FANCM) patients. FA is a genomic instability disorder clinically characterized by congenital abnormalities, progressive bone marrow failure, and predisposition to malignancy. The FA core complex consists of fourteen proteins participating in a DNA damage response network with breast cancer susceptible proteins, BRCA1 and BRCA2. Based on homology between MPH1 and FANCM, we hypothesized yeast also has a FA like pathway that functions for repair of intercrosslink (ICL) repair. ICLs covalently link complementary DNA strands, block DNA replication and transcription, and must be removed to allow cell survival. We genetically characterized a conserved yeast ICL repair pathway comprised of the yeast homologs (Mph1, Chl1, Mhf1, Mhf2) of four FA proteins (FANCM, FANCJ, MHF1, MHF2). We found that this pathway is epistatic with Rad5-mediated DNA damage bypass and distinct from the ICL repair pathways mediated by Rad18 and Pso2 that remove ICL damage by different mechanisms. In addition, consistent with FANCMs role in stabilizing ICL-stalled replication forks, we found that Mph1 prevents ICL-stalled replication forks from collapsing into double strand breaks. This unique repair function of Mph1 is specific for ICL damage and does not extend to other types of damage. These studies reveal the functional conservation of the FA pathway and validate the yeast model for future studies to further elucidate the mechanism of the FA pathway. Whole studies are reported in Journal of Biological Chemistry. Due to the departure of post-doctoral fellow who studied this project, the Mph1 project is now closed.

在不造成有害基因改变的情况下传递遗传信息是最重要的任务之一。细胞已经进化出了检测和修复潜在致命DNA损伤的系统。然而，当这些系统不能正常工作时，DNA损伤就会累积并导致基因变化或细胞死亡。遗传变化的积累被定义为基因组的不稳定性，在包括癌症在内的各种类型的遗传疾病中经常观察到。基因组不稳定已被证明是肿瘤抑制基因多次失活和原癌基因激活的前一步。在许多癌症中经常观察到的一种基因组不稳定性是染色体重排（GCR）。GCR包括易位、染色体臂缺失、间质缺失、倒位、扩增、染色体端到端融合和非整倍体。尽管对癌细胞中观察到的GCRs的起源和机制知之甚少，但最近对遗传性癌症易感综合征中突变基因的研究已经开始表明，在DNA损伤反应、DNA修复和DNA重组中起作用的蛋白质在抑制自发和/或DNA损伤诱导的GCRs中起着至关重要的作用。最近对包括癌症在内的遗传性疾病的基因与GCRs之间的强相关性的鉴定开始确定GCRs的重要性。为了了解GCR的产生机制以及GCR的形成如何导致肿瘤发生，我们筛选了整个酵母基因组中增加GCR形成率的突变或过表达。我们选择了来自突变筛选的RAD5和ELG1以及来自过表达筛选的MPH1来进一步研究这些蛋白保护基因组免受有害GCR形成的分子机制。