FUNCTIONAL CHARACTERIZATION OF KINETOCHORE PROTEIN CNN1P

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 Cnn1p是最近在已知动粒成分的免疫沉淀物中发现的一种新的酿酒酵母动粒蛋白。Cnn1p是一种非必需蛋白，其从细胞中消除不会产生任何表型(染色体丢失、纺锤体检查点激活等)。Cnn1p的表达量非常低，这使得它的纯化变得极其困难。通过全基因组双杂交筛选，我们希望找到Cnn1p的合作伙伴，这将有助于更好地理解Cnn1p在染色体分离过程中所扮演的角色。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Cnn1p is a novel S. cerevisiae kinetochore protein that was recently identified in the immunoprecipitates of known kinetochore components. Cnn1p is a non-essential protein whose elimination from the cell gives no phenotypes (chromosome loss, spindle checkpoint activation, etc.). Cnn1p is expressed in very low amounts making its purification extremely difficult. Via a whole-genome two-hybrid interaction screen we hope to identify partners of Cnn1p which will lead to a better understanding of the roles that Cnn1p carries out during chromosome segregation.