FUNCTIONAL CHARACTERIZATION OF KINETOCHORE PROTEIN CNN1P

该子项目是利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得主要资金， 因此可以在其他CRISP条目中表示。列出的机构是 中心，不一定是研究者的机构。 Cnn 1 p是一个新的S.酿酒酵母动粒蛋白，最近在已知动粒组分的免疫沉淀物中鉴定。Cnn 1 p是一种非必需蛋白质，从细胞中消除不会产生表型（染色体丢失，纺锤体检查点激活等）。Cnn 1 p的表达量非常低，使得其纯化极其困难。通过全基因组双杂交互作筛选，我们希望确定Cnn 1 p的合作伙伴，这将导致更好地了解Cnn 1 p在染色体分离过程中的作用。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Cnn1p is a novel S. cerevisiae kinetochore protein that was recently identified in the immunoprecipitates of known kinetochore components. Cnn1p is a non-essential protein whose elimination from the cell gives no phenotypes (chromosome loss, spindle checkpoint activation, etc.). Cnn1p is expressed in very low amounts making its purification extremely difficult. Via a whole-genome two-hybrid interaction screen we hope to identify partners of Cnn1p which will lead to a better understanding of the roles that Cnn1p carries out during chromosome segregation.