COPPER SPATIAL DISTRIBUTION, CONCENTRATION & STATUS IN NORMAL & TUMOR TISSUES

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目及 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 在先前的实验中，我们已经证明DSF和CQ可以与溶液中的Cu相互作用，形成新的化合物。组合的CQ-Cu和DSF-Cu化合物可以用作具有最小毒性水平的抗癌药物。为了研究Cu在正常和肿瘤组织中的分布，我们对来自小鼠乳腺和小鼠前列腺组织的正常和肿瘤组织进行元素荧光映射。我们还在处理的组织中进行了定位实验，以研究上述化合物在体内Cu分布的影响。使用的样品是乳腺癌和前列腺癌组织。我们的数据表明，Cu在正常组织中含量很低，在肿瘤组织中含量较高。我们还测量了两组样品中Cu，Zn和Fe分布的相关性。结果表明化合物对肿瘤组织样品具有显著的活性。更多的样本正在准备中，以研究药物在肿瘤发展的不同阶段的作用。实验条件的优化也在开发中。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. In a previous experiment, we have demonstrated that DSF and CQ can interact with Cu in solution forming a new compound. The combined CQ-Cu and DSF-Cu compounds can be used as anticancer drugs with minimum toxicity levels. To investigate the Cu distribution in normal and tumor tissues we performed elemental fluorescence mapping on normal and tumor tissue from mouse breast and mouse prostate tissue. We also performed mapping experiment in treated tissues to investigate the effect of the mentioned compounds in the Cu distribution in vivo. The samples used were breast cancer and prostate cancer tissue. Our data indicate that Cu is found in very low amount in normal tissue and in higher levels in tumor tissue. We have also measured the correlation of Cu, Zn and Fe distributions in both sets of samples. The results indicate a significant activity of the compounds at the tumor tissue samples. More samples are under preparation to investigate the effect of the drugs at different stages of the tumor development. Optimization of the experimental conditions is also under development.