CANDIDA ALBICANS-ASSOCIATED ORAL BIOFILMS

白色念珠菌相关口腔生物膜

• 批准号: 7610834
• 负责人: Caroline Westwater
• 金额: $ 14.3万
• 依托单位: MEDICAL UNIVERSITY OF SOUTH CAROLINA
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-08-01 至 2008-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7610834
• 关键词: Accounting; Architecture; Binding; Biological; Biology; Candida; Candida albicans; Candidiasis; Cells; Clinical; Communities; Computer Retrieval of Information on Scientific Projects Database; Denture Stomatitis; Development; Extracellular Matrix; Funding; Glycoproteins; Goals; Grant; Growth; Heterogeneity; Human; Infection; Institution; Knowledge; Maintenance; Microbial Biofilms; Modeling; Morbidity - disease rate; Oral cavity; Patients; Periodontal Diseases; Phenotype; Play; Population; Production; Public Health; Refractory; Research; Research Personnel; Resources; Role; Site; Source; Streptococcus; Streptococcus gordonii; Surface; United States National Institutes of Health; antimicrobial; base; candida biofilm; mannoproteins; microbial disease; mortality; oral bacteria; oral biofilm; pathogen

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Candida species are the major fungal pathogen of humans and are responsible for significant morbidity and mortality. Candidiasis is often associated with the formation of Candida biofilms on the surface of inanimate or biological surfaces, and this phenotype is found at both mucosal and systemic sites. Biofilm-based infections are estimated to account for as much as 65% of all human microbial diseases, are frequently refractory to conventional antimicrobial therapy and are a source of recalcitrant infections; consequently, biofilm-associated infections represent a significant public health problem. Several artificial biofilm models have shown that mature Candida biofilms display spatial heterogeneity and a layered architecture consisting of extracellular matrix encased microcolonies. It should be emphasized, however, that clinical biofilms rarely, if ever, consist of only cells from a single species. Indeed interactions between oral bacteria and Candida such as coaggregation, coadhesion, growth stimulation or inhibition most likely play an important role in the development and maintenance of mixed-microbial biofilms. The long-term goal of this research is to understand the biology of Candida albicans biofilm formation in the oral cavity and to dissect the numerous interactions between fungal and bacterial species within a biofilm community. Specifically, we will 1) determine if Candida mannoproteins are important for exopolysaccharide matrix production, 2) study the development of a mixed-species biofilm consisting of oral bacteria (streptococci) and C. albicans, and 3) identify and analyze C. albicans glycoproteins capable of binding Streptococcus gordonii. By studying mixed-species biofilm formation and applying this knowledge to the patient population, we will gain a better understanding of many biofilm-based infections including denture stomatitis and periodontal disease.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 念珠菌属是人类的主要真菌病原体，并导致显著的发病率和死亡率。 念珠菌病通常与无生命或生物表面上念珠菌生物膜的形成有关，并且这种表型在粘膜和全身部位都有发现。 据估计，基于生物膜的感染占所有人类微生物疾病的65%，通常对常规抗微生物治疗无效，并且是难治性感染的来源;因此，生物膜相关感染是一个重大的公共卫生问题。 几种人工生物膜模型已经表明，成熟的念珠菌生物膜显示空间异质性和由细胞外基质包裹的小菌落组成的分层结构。 然而，应该强调的是，临床生物膜很少（如果有的话）仅由来自单一物种的细胞组成。 事实上，口腔细菌和念珠菌之间的相互作用，如共聚集，共粘附，生长刺激或抑制最有可能在混合微生物生物膜的发展和维持中发挥重要作用。 本研究的长期目标是了解口腔中白色念珠菌生物膜形成的生物学，并剖析生物膜群落中真菌和细菌物种之间的众多相互作用。 具体来说，我们将1）确定念珠菌甘露糖蛋白是否对胞外多糖基质的产生很重要，2）研究口腔细菌（链球菌）和念珠菌组成的混合物种生物膜的发展。白色念珠菌; 3）对念珠菌进行鉴定和分析。能够结合戈登链球菌的白色念珠菌糖蛋白。通过研究混合物种生物膜形成并将这些知识应用于患者群体，我们将更好地了解许多基于生物膜的感染，包括义齿性口炎和牙周病。