BIOSYNTHESIS OF ISOFLAVONOID AND FLAVONOID NUTRIENTS

异黄酮和类黄酮营养素的生物合成

• 批准号: 7610258
• 负责人: Nancy Paiva
• 金额: $ 7.79万
• 依托单位: UNIVERSITY OF OKLAHOMA HLTH SCIENCES CTR
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-05-01 至 2008-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7610258
• 关键词: Alfalfa; Anabolism; Animal Feed; Animals; Anthocyanins; Area; Biological; Biological Availability; Biological Factors; Biomedical Research; Catechin; Chemicals; Computer Retrieval of Information on Scientific Projects Database; Condition; Copper Sulfate; Diet; Environment; Enzymes; Escherichia coli; Factor Analysis; Flavonoids; Funding; Future; Gene Expression; Genes; Glucosides; Goals; Grant; Institution; Measures; Medicago truncatula; Nutrient; Oligonucleotides; Pathway interactions; Pichia; Plants; Proanthocyanidins; RNA; Reagent; Relative (related person); Research; Research Personnel; Resources; Reverse Transcriptase Polymerase Chain Reaction; Sampling; Seedling; Seeds; Source; Testing; Tissues; United States National Institutes of Health; Variant; base; beta Glucosidases; beta-Glucosidase; enzyme pathway; expression cloning; gene discovery; improved; laboratory facility; microbial; mutant; nutrition; research study; vector

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The long term goal of this research is to identify and characterize clones encoding biosynthetic enzymes essential to the accumulation of beneficial natural products in plants. These clones may later be used to analyze factors which effect the accumulation of these compounds in plants, to improve their availability in crop species, or to facilitate biomedical nutrition and bioavailability studies. The main natural product areas are anthocyanins, catechins/condensed tannins, and isoflavonoids. Initially, much effort involved acquiring and propagating necessary biological reagents, including mutant plant lines accumulating visibly different levels of natural products, and re-organizing laboratory facilities from strictly chemical research to a more biomedical research environment. Vectors for microbial over-expression of cloned Medicago truncatula beta-glucosidases active on isoflavonoid glucosides have been constructed, and are being tested in E. coli and Pichia pastoris, with the goal of producing and purifying sufficient enzyme to test in animal feeding studies. Condensed tannins from seeds from alfalfa mutants will be analyzed, for comparison with gene expression analysis. Conditions for induction of isoflavonoid pathway enzymes in M. truncatula seedlings using copper sulfate will be investigated. RT-PCR primers will be used to measure the relative expression levels of known flavonoid and isoflavonoid pathway genes in induced tissues or in tissues from mutant and wild-type plant lines. Satisfactory RNA samples will be probed using 70-mer oligo arrays developed for M. truncatula. In addition to revealing the gene-expression differences conferring the natural product variations in mutant lines, these experiments should facilitate future microarray-based gene discovery or animal diet analysis.

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