CONSERVATION GENETICS OF FINESCALE DACE: DEVELOPMENT OF MICROSATELLITE MARKERS

细鳞鲮鱼的保护遗传学：微卫星标记的开发

• 批准号: 7610297
• 负责人: CYNTHIA M ANDERSON
• 金额: $ 6.91万
• 依托单位: UNIVERSITY OF SOUTH DAKOTA
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-05-01 至 2008-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7610297
• 关键词: Computer Retrieval of Information on Scientific Projects Database; Dace; Development; Funding; Future; Genetic; Genetic Polymorphism; Genomic Library; Grant; Institution; Libraries; Microsatellite Repeats; Nucleotides; Polymerase Chain Reaction; Population Genetics; Research; Research Personnel; Resources; Source; United States National Institutes of Health; design

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Jake Miller will construct a genomic library for the Finescale Dace, Phoxinus neogaeus, enriched for microsatellite sequences. This library will be screened for the presence of di-, tri- and tetra-nucleotide repeat regions that may be used as microsatellite markers. Those regions will be sequenced and PCR primers specific to those regions will be designed and optimized. Once optimized the primers will be available to be used in a study to assess the amount of polymorphism at each locus and the utility of the microsatellite markers in future population genetics.

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