SURVEY OF PRION GENE SEQUENCES OF WILD ANIMALS IN BLACK HILLS

黑山野生动物朊病毒基因序列调查

• 批准号: 7381688
• 负责人: CYNTHIA M ANDERSON
• 金额: $ 6.09万
• 依托单位: UNIVERSITY OF SOUTH DAKOTA
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-05-01 至 2007-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7381688
• 关键词: SURVEY; PRION; GENE; SEQUENCES; WILD

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This primary goal of this project is to identify the sequences of the prion protein gene (PRNP) in various animals. The two main areas of interest are the evolutionary history of the gene and possible serial transmission. The main focus thus of this project is to sequence PRNP from carnivores and other animals common to the Black Hills region. The reason carnivores were chosen is because of the real possibility of them consuming the flesh of animals that have been documented to contract this progressive, fatal disease. We have obtained existing tissue samples from area trappers, law enforcement officers and taxidermists, and have extracted DNA from the following animals; coyote, badger, raccoon, bobcat, lynx, skunk and pronghorn antelope. The PRNP coding region has been sequenced from each of these animals using degenerate primer sets to obtain partial coding sequence. New species specific primers will be designed in order to fully sequence the coding regions of each of these animals. Additionally, an attempt will be made to obtain sequence data for certain areas of the promoter region of the PRNP gene. All sequence data will be compared to a national database of previously sequenced animals in hopes of identifying similarities that would give some insight to the evolutionary history of the gene as well as the possibility of serial transmission.

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。这个项目的主要目标是确定不同动物的PrNP基因序列。感兴趣的两个主要领域是基因的进化史和可能的连续传播。因此，该项目的主要重点是对黑山地区常见的食肉动物和其他动物的PRNP进行测序。之所以选择食肉动物，是因为它们确实有可能食用动物的肉，而这些动物已经被记录为感染了这种进行性的致命疾病。我们已经从捕猎者、执法人员和标本制作者那里获得了现有的组织样本，并从以下动物身上提取了DNA：郊狼、獾、浣熊、山猫、臭鼬和叉角羚。用简并的引物对这些动物的PRNP编码区进行了测序，得到了部分编码序列。将设计新的物种特异性引物，以便对每种动物的编码区进行完全测序。此外，还将尝试获得PRNP基因启动子区域某些区域的序列数据。所有的序列数据将与以前测序的动物的国家数据库进行比较，希望确定相似性，这将为基因的进化史以及连续传播的可能性提供一些洞察。