AFFECTS OF AG COMPOUNDS ON METAL TRANSPORT GENES IN DERMATOPHYTIC FUNGI

AG 化合物对皮肤癣菌金属转运基因的影响

• 批准号: 8167992
• 负责人: CYNTHIA M ANDERSON
• 金额: $ 9.55万
• 依托单位: UNIVERSITY OF SOUTH DAKOTA
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-01 至 2011-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8167992
• 关键词: Affect; Area; Arthrodermataceae; Bacteria; Burn injury; Candida; Candida albicans; Cloning; Computer Retrieval of Information on Scientific Projects Database; Funding; Gene Targeting; Genes; Genetic; Goals; Grant; Growth; Institution; Metals; Microbe; Microbial Biofilms; Microsporidia; Microsporum; Patients; Play; Research; Research Personnel; Resistance; Resources; Role; Silver; Silver Compounds; Silver Nitrate; Source; Testing; Time; Trichophyton; United States National Institutes of Health; design; fungus; interest; research study; resistance mechanism; tool; wound

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Dermatophytic fungi have been known to colonize wound biofilms. Current treatment of burn patients involve covering the area with silver nitrate, or colloidal silver impregnated patches to inhibit the growth of microbes in wound biofilms. While information on sensitivity, mode of action and mechanism of resistance is readily available for bacteria, little is available for dermatophytic fungi. The goal of this project is to understand the mechanism of tolerance or resistance to silver compounds in dermatophytic fungi. We have determined the MIC of silver nitrate against Candida albicans, Candida krusei, Trichophyton mentagrophytes, and Microsporum gypseum. Our research is now focusing on completing the design and optimization of qPCR primers for genes hypothesized to play a role in silver tolerance in Candida albicans. Real-time qPCR experiments to test changes in expression of the chosen target genes is being done at various levels up to and just over the MIC. If possible, the same experiments will be done in the filamentous dermatophytes Trichophyton mentagrophytes, and Microsporum gypseum. However, the lack of genetic information for these species and the lack of similarity between species at the targeted loci presents a challenge. The results of our experiments with C. albicans will help us to target the loci of interest for isolation, cloning and sequencing in the filamentous dermatophytes. This will aid in our quest to develop the genetic tools required for studying silver tolerance and resistance in the filamentous dermatophytes.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 已知皮肤真菌在伤口生物膜上定殖。 目前对烧伤患者的治疗涉及用硝酸银或胶体银浸渍贴片覆盖该区域以抑制伤口生物膜中微生物的生长。 虽然关于细菌的敏感性、作用方式和耐药机制的信息很容易获得，但对于皮肤真菌却很少。 本计画的目的是了解皮肤癣菌对银化合物产生耐受或抗性的机制。我们测定了银对白色念珠菌、克柔念珠菌、须癣毛癣菌和石膏样小孢子菌的MIC。我们的研究现在集中在完成qPCR引物的设计和优化，假设在白色念珠菌的银耐受性中发挥作用的基因。 实时qPCR实验测试所选靶基因表达的变化，在高达和刚好超过MIC的各种水平下进行。如果可能，将在丝状皮肤真菌须毛癣菌和石膏样小孢子菌中进行相同的实验。 然而，缺乏这些物种的遗传信息以及物种之间在目标基因座处缺乏相似性提出了挑战。我们的实验结果与C.白色念珠菌的基因将帮助我们在丝状皮肤真菌中靶向感兴趣的基因座进行分离、克隆和测序。 这将有助于我们寻求开发研究丝状皮肤真菌中银耐受性和抗性所需的遗传工具。