GENETIC MECHANISM OF INTERSPECIES COMMUNICATION BETWEEN DENTAL PLAQUE BACTERIA

牙菌斑细菌种间通讯的遗传机制

• 批准号: 7610320
• 负责人: Paul Egland
• 金额: $ 1.48万
• 依托单位: UNIVERSITY OF SOUTH DAKOTA
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-05-01 至 2008-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7610320
• 关键词: Amylases; Bacteria; Cells; Communication; Communities; Computer Retrieval of Information on Scientific Projects Database; Dental Plaque; Development; Funding; Genes; Genetic; Goals; Grant; Human; Institution; Metabolic; Microbial Biofilms; Molecular; Mutation; Organism; Play; Research; Research Personnel; Resources; Role; Signal Transduction; Source; Streptococcus gordonii; United States National Institutes of Health; Veillonella atypica; Work; alpha-amylase; base; interest; member; mutant; oral biofilm; response

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The human oral biofilm, dental plaque, is a well-recognized example of a multi-species bacterial community. My research interest lies in the study of communication between different species of bacteria that compose dental plaque. This communication includes cell-cell contact and metabolic interactions between organisms in the biofilm. My current research focus is communication that occurs between two members of the plaque community, Veillonella atypica and Streptococcus gordonii. When these organisms are growing together, S. gordonii induces expression of an alpha-amylase gene that is not expressed when S. gordonii is grown without V. atypica. My goals are to identify the mechanism of signaling that occurs between these species and determine the importance of amylase expression to development of the mixed-species community. We have recently identified a gene that may play a role in the ability of S. gordonii to induce amylase expression in response to V. atypica. As a first step in understanding the molecular basis for the signalling between S. gordonii and V. atypica, we constructed a strain carrying a mutation in this gene. Current work is aimed at characterizing the mutant with respect to its ability to respond to V. atypica.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 人类口腔生物膜（牙菌斑）是多物种细菌群落的一个公认的例子。 我的研究兴趣在于研究构成牙菌斑的不同种类细菌之间的通讯。 这种通讯包括生物膜中生物体之间的细胞间接触和代谢相互作用。 我目前的研究重点是斑块群落的两个成员（非典型韦荣球菌和戈登链球菌）之间发生的交流。 当这些生物体一起生长时，戈登沙门氏菌会诱导α-淀粉酶基因的表达，而当戈登沙门氏菌在没有非典型弧菌的情况下生长时，该基因不会表达。 我的目标是确定这些物种之间发生的信号传导机制，并确定淀粉酶表达对混合物种群落发展的重要性。 我们最近发现了一个基因，该基因可能在戈登沙门氏菌诱导淀粉酶表达以响应非典型弧菌的能力中发挥作用。 作为了解戈登沙门氏菌和非典型弧菌之间信号传导分子基础的第一步，我们构建了一种携带该基因突变的菌株。 目前的工作旨在表征突变体对非典型弧菌的反应能力。