INTERSPECIES BACTERIAL INTERACTIONS IN HUMAN DENTAL PLAQUE

人类牙菌斑中的种间细菌相互作用

• 批准号: 8168005
• 负责人: Paul Egland
• 金额: $ 2.5万
• 依托单位: UNIVERSITY OF SOUTH DAKOTA
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-01 至 2011-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8168005
• 关键词: Amylases; Bacteria; Child; Communication; Communities; Computer Retrieval of Information on Scientific Projects Database; Dental Plaque; Development; Funding; Genes; Goals; Grant; Human; Institution; Metabolic; Microbial Biofilms; Native Americans; Oral; Oral health; Organism; Research; Research Personnel; Resources; Signal Transduction; Source; Streptococcus gordonii; Surveys; Underserved Population; United States National Institutes of Health; Veillonella atypica; alpha-amylase; interest; member; response; transcription factor

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Human dental plaque is a well-recognized example of a multi-species bacterial community. My research interest lies in the interaction between different bacterial species found in dental plaque and the study of communication between different species of bacteria in the plaque community. This communication includes metabolic interactions between organisms in the biofilm. My current research focus includes studies of communication that occurs between two members of the plaque community, Veillonella atypica and Streptococcus gordonii. When these organisms are growing together, S. gordonii induces expression of an alpha-amylase gene that is not expressed when S. gordonii is grown without V. atypica. My goals are to identify the mechanism of signaling that occurs between these species and determine the importance of amylase expression to development of the mixed-species community. I have recently identified a transcription factor is required for S. gordonii to induce amylase expression in response to V. atypica. I plan to identify other genes required for this signaling. In addition, I am currently expanding my research to include a survey the species of oral bacterial present in Native American children, an underserved population with disparities in oral health.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 人类牙菌斑是一个公认的多物种细菌群落的例子。 我的研究兴趣在于牙菌斑中发现的不同细菌物种之间的相互作用，以及牙菌斑群落中不同细菌物种之间的交流研究。 这种交流包括生物膜中生物体之间的代谢相互作用。 我目前的研究重点包括两个成员之间发生的斑块社区，韦荣氏球菌和链球菌戈登的通信的研究。 当这些生物生长在一起时，S。gordonii诱导α-淀粉酶基因的表达，该基因在S. gordonii在没有V. 我的目标是确定这些物种之间发生的信号传导机制，并确定淀粉酶表达对混合物种群落发展的重要性。 我最近发现了一个转录因子是S。gordonii诱导淀粉酶的表达。 我计划鉴定这种信号传导所需的其他基因。 此外，我目前正在扩大我的研究范围，包括调查美国土著儿童中存在的口腔细菌种类，这是一个口腔健康差距悬殊的服务不足的人群。