Interferon regulation of T-bet in airway smooth muscle

干扰素对气道平滑肌 T-bet 的调节

• 批准号: 7269459
• 负责人: Cherie Singer
• 金额: $ 12.27万
• 依托单位: UNIVERSITY OF NEVADA RENO
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-09-30 至 2009-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7269459
• 关键词: Address; Affect; Award; Biology; Boxing; CD4 Positive T Lymphocytes; Cell Cycle Progression; Cell Proliferation; Cellular biology; Commit; Contractile Proteins; Development; Development Plans; Disease; Educational process of instructing; Educational workshop; Environment; Event; Faculty; Foundations; Goals; Helper-Inducer T-Lymphocyte; Inflammatory; Inflammatory Response; Interferon Type I; Interferon Type II; Interferons; K-Series Research Career Programs; Laboratories; Mediating; Mediator of activation protein; Mentors; Muscle Contraction; Neurosciences; Nevada; Phenotype; Postdoctoral Fellow; Protein Overexpression; Regulation; Research; Research Personnel; Research Project Grants; Research Training; Role; Services; Signal Pathway; Smooth Muscle; Smooth Muscle Myocytes; Structure; Students; T-bet protein; Testing; Thinking; Universities; career; cytokine; experience; innovation; instructor; interest; medical schools; migration; novel; oral communication; programs; protein expression; research and development; research study; respiratory smooth muscle; response; skills

DESCRIPTION (provided by applicant): A Mentored Career Development Award will allow me to become an innovative, recognized investigator and instructor by expanding my research skills, enhancing my ideas for my own research program in airway smooth muscle biology, and providing me with opportunities to continue to develop expertise in teaching through the outlined career development and research plans. I believe that my previous training and research experience at both large and small universities has given me an excellent foundation in which to begin an independent research career. As a graduate student, I endured a rigorous program of study that emphasized critical and analytical thinking, oral communication and presentation skills, and research excellence in the neurosciences. As a post-doctoral fellow, I developed an interest in the role of airway smooth muscle (ASM) in inflammatory events that contribute to airway diseases and took advantage of opportunities to gain teaching experience. I continue my interest in airway smooth muscle biology with this proposal by examining the T-box transcription factor T-bet in ASM. The proposed career development plan will facilitate my goals through intellectual interactions with colleagues, students and my mentor, Dr. William T. Gerthoffer, along with participation in structured activities including University service, attendance at scientific meetings, and involvement in teaching, career development and laboratory management workshops and seminars. The research project described here examines the hypothesis that ASM expression of T-bet participates in the development of a secretory smooth muscle cell phenotype that affects the inflammatory response by increasing T helper cell 1 (Th1) cytokine secretion. The experiments described will examine IFNgamma regulation of T-bet expression in ASM, determine whether T-bet expression contributes to the development of a secretory smooth muscle cell phenotype by upregulating Th1 cytokines, and addresses whether T-bet alters the functions of ASM cells such as proliferation, migration and contraction. There are few places that I would have the opportunity to interact with faculty that are as recognized for their expertise in smooth muscle cell biology than at the University of Nevada School of Medicine. This award will allow me to begin to ask novel questions about the secretory phenotype of ASM in this supportive research environment that is committed to furthering junior faculty development.

描述(由申请人提供)： 导师职业发展奖将通过扩展我的研究技能，增强我对自己的呼吸道平滑肌生物学研究项目的想法，并为我提供机会，通过概述的职业发展和研究计划，继续发展教学专业知识，使我成为一名创新的、得到认可的研究人员和教师。我相信，我之前在大大小小大学的培训和研究经验，给了我开始独立研究生涯的良好基础。作为一名研究生，我经历了严格的学习计划，强调批判性和分析性思维，口头交流和陈述技能，以及神经科学方面的卓越研究。作为一名博士后研究员，我对呼吸道平滑肌(ASM)在导致呼吸道疾病的炎症事件中的作用产生了兴趣，并利用机会获得教学经验。我通过检测ASM中的T-box转录因子T-bet来继续我对呼吸道平滑肌生物学的兴趣。拟议的职业发展计划将通过与同事、学生和我的导师William T.Gerthoffe博士进行智力互动，以及参与有组织的活动，包括为大学服务、参加科学会议，以及参与教学、职业发展和实验室管理研讨会和研讨会，来促进我的目标。本文介绍的研究项目验证了T-bet的ASM表达参与分泌的平滑肌细胞表型的发展，该表型通过增加T辅助细胞1(Th1)细胞因子的分泌来影响炎症反应的假设。所描述的实验将检测IFNGamma对ASM中T-bet表达的调节，确定T-bet的表达是否通过上调Th1细胞因子而促进分泌性平滑肌细胞表型的发展，以及T-bet是否改变ASM细胞的功能，如增殖、迁移和收缩。在内华达大学医学院，我很少有机会与在平滑肌细胞生物学方面被公认为专业的教职员工互动。这个奖项将允许我开始提出新的问题，在这个致力于促进初级教师发展的支持性研究环境中，关于ASM的分泌表型。