MECHANISMS OF ALLERGIC SENSITIZATION BY NITROGEN DIOXIDE EXPOSURE

二氧化氮暴露引起过敏的机制

• 批准号: 7720874
• 负责人: Matthew E Poynter
• 金额: $ 23.86万
• 依托单位: UNIVERSITY OF VERMONT & ST AGRIC COLLEGE
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-05-01 至 2009-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7720874
• 关键词: Air Pollutants; Allergic; Asthma; Breathing; Breeding; Cell Surface Receptors; Cells; Computer Retrieval of Information on Scientific Projects Database; Disease; Epithelial Cells; Epithelium; Event; Funding; Gases; Grant; Immune; Institution; Knockout Mice; Ligands; Lung; Mus; Nitrogen Dioxide; Numbers; Patients; Play; Research; Research Personnel; Resources; Role; Signal Transduction; Signaling Molecule; Source; Testing; Tube; United States National Institutes of Health; airway epithelium; design; drug development; insight; mouse model; prevent; receptor; research study; response

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Asthma is a disease characterized by the presence of immune cells, which are capable of damaging the lungs and contributing to the breathing difficulty of patients. One molecule formed by these immune cells is the toxic gas, nitrogen dioxide (NO2), more commonly known as an air pollutant. It is completely unclear whether NO2 contributes to asthma and how it acts on the lung. Since lung epithelial cells, which line the breathing tube, may come in contact with a considerable amount of NO2, and also are believed to play an important role in asthma, the focus of Project 3 is to examine the consequences of NO2-induced damage to the lung epithelial cells. We have designed experiments in intact mice, which can be exposed to NO2 via inhalation. Our objective is to test whether cell signaling events are important in the pathological response to NO2 inhalation and whether they may contribute to asthma exacerbation and sensitization. We have determined that NO2 inhalation causes damage to the lung epithelium and induces the release of intracellular molecules, which are normally sequestered away from recognition by cell surface receptors. Candidate receptors for these intracellular molecules are expressed by airway epithelium and induce intracellular signaling cascades when engaged by ligands. We are determining the role of these receptors, as well as intracellular signaling molecules, in the exacerbation of asthma in a mouse model by inhalation of NO2. To do so, we have obtained knockout mice from collaborators and are currently breeding sufficient numbers of mice for our studies. These studies will provide an important insights into the mechanisms by which toxic agents released by immune cells can cause damage to the lung, and may result in the development of drugs to prevent that damage from occurring.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 哮喘是一种以免疫细胞的存在为特征的疾病，免疫细胞能够损害肺部并导致患者呼吸困难。由这些免疫细胞形成的一种分子是有毒气体，二氧化氮（NO2），通常被称为空气污染物。目前还不清楚NO2是否会导致哮喘，以及它如何作用于肺部。由于肺上皮细胞，内衬呼吸管，可能会接触到大量的NO2，也被认为是在哮喘中发挥重要作用，项目3的重点是检查NO2诱导的肺上皮细胞损伤的后果。 我们设计了完整的小鼠实验，可以通过吸入暴露于NO2。 我们的目的是测试细胞信号事件是否在NO2吸入的病理反应中是重要的，以及它们是否可能导致哮喘恶化和致敏。 我们已经确定，吸入NO2会导致肺上皮细胞损伤，并诱导细胞内分子的释放，这些分子通常被隔离，无法被细胞表面受体识别。 这些细胞内分子的候选受体由气道上皮表达，并且当被配体接合时诱导细胞内信号级联。 我们正在确定这些受体以及细胞内信号分子在吸入NO2的小鼠哮喘加重模型中的作用。 为此，我们从合作者那里获得了基因敲除小鼠，目前正在为我们的研究繁殖足够数量的小鼠。 这些研究将为免疫细胞释放的有毒物质对肺部造成损害的机制提供重要见解，并可能导致药物的开发，以防止这种损害的发生。