Multiscale Engineering of Cell Scaffold for Bone Regeneration

骨再生细胞支架的多尺度工程

• 批准号: 7628955
• 负责人: Nicholas Alexander Kotov
• 金额: $ 31.88万
• 依托单位: UNIVERSITY OF MICHIGAN AT ANN ARBOR
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-09-01 至 2011-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7628955
• 关键词: Adhesions; Affect; Architecture; Biological; Bone Growth; Bone Regeneration; Bone Tissue; Bone Transplantation; Caliber; Cell Adhesion; Cell Culture Techniques; Cell Density; Cell-Matrix Junction; Chemistry; Computers; Controlled Environment; Cultured Cells; Defect; Degenerative Disorder; Development; Dimensions; Disease; Engineering; Environment; Generations; Goals; Growth; Human; Hydroxyapatites; Immune; Implant; Infection; Injury; Life; Mechanics; Military Personnel; Modification; Molds; Morphology; Natural regeneration; Nature; Nutrient; Operative Surgical Procedures; Osteoblasts; Performance; Polymers; Porosity; Predisposition; Preparation; Principal Investigator; Process; Property; Protocols documentation; Research; Risk; SCID Mice; Shapes; Solutions; Sports; Structure; Surface; Surface Properties; Techniques; Testing; Tissue Donors; Tissue Engineering; Tissues; Trauma; War; Weight-Bearing state; age related; allogenic bone transplantation; base; bone; bone cell; calcium phosphate; calcium phosphate coating; cell growth; cell motility; cellular engineering; computer design; design; improved; in vivo; migration; nanocomposite; nanoparticle; nanoscale; programs; prototype; scaffold

DESCRIPTION (provided by applicant): Bone implants are in great demand for the treatment of for victims of trauma, military actions, congenital and degenerative diseases, as well as sports- and age-related injuries. The need for replacement tissue far outweighs the availability of donor tissue. The deficit is further exacerbated by war injuries. In this project we will target engineering of artificial bone implants that can potentially provide an endless supply of the bone material and eliminate the risk of immune rejection or disease transfer. Borrowing the concept from Nature, we propose to take advantage of multi-scale design of the bone scaffolds spanning the scale from 10-8 to 10-1 m. Multiple requirements of the artificial bone tissue, that include both mechanical and biological properties, make this approach acutely necessary. The following Specific Aims will be pursued to produce and implant prototype sustaining rapid development of bone cells, as well as high compression strengths. (1). Preparation of a biodegradable nanocomposite of PLGA and calcium phosphate (CP) nanoparticles with improved mechanical properties (10-8-10-6 m scale); (2). Construction of 3D biodegradable scaffold structure with inverted colloidal crystal (ICC) topography (10-4-10-6 m scale). (3) Increased cell attachment and growth by optimizing surface roughness through the application of nanoparticles to the scaffold surface (10-8-10-4 m scale); (4). Preparation of ICC scaffolds from PLGA-CP composite with a computer-designed cross-bar geometry (10-3-10-1 m scale); and (5). In vivo testing of the integrated scaffold with optimal geometry, mechanical properties, and surface roughness. These Specific Aims will allow us to obtain prototypes of implantable scaffolds, identify potential challenges and prove or disprove the principal concept of multi-scale design for artificial bone.

描述（由申请人提供）：骨植入物非常需要为创伤，军事行动，先天性和退化性疾病以及运动和年龄相关的伤害的受害者治疗。需要替换组织的需求远远超过供体组织的可用性。战争受伤进一步加剧了赤字。在这个项目中，我们将针对人造骨植入物的工程，该工程可能会提供无限的骨料供应并消除免疫排斥或疾病转移的风险。借用自然界的概念，我们提议利用骨支架的多尺度设计，跨度从10-8到10-1 m。人造骨组织的多种要求，包括机械性能和生物学特性，使这种方法绝对必要。将追求以下特定目标，以生产和植入骨细胞快速发展以及高压缩强度的原型。 （1）。具有改善机械性能的PLGA和磷酸钙（CP）纳米颗粒的可生物降解纳米复合材料（10-8-10-6 m比例）； （2）。具有倒置胶体晶体（ICC）地形（10-4-10-6 m尺度）的3D可生物降解支架结构的结构。 （3）通过将纳米颗粒应用于脚手架表面（10-8-10-4 m等级）来优化表面粗糙度，从而增加了细胞的附着和生长； （4）。用计算机设计的跨杆几何形状（10-3-10-1 m比例）从PLGA-CP复合材料中制备ICC支架； （5）。具有最佳几何形状，机械性能和表面粗糙度的集成支架的体内测试。这些特定的目标将使我们能够获得可植入的支架的原型，确定潜在的挑战，并证明或反驳人造骨多尺度设计的主要概念。