Task Specific Project 2

具体任务项目2：用于术中指导的FDPM手术器械 此特定任务项目的目标是使我们的FDPM器械用于外科套件，以定位 并鉴定通过与靶向部分连接的近红外染料描绘的癌症阳性LN 主要项目。在第三年，将开发第一台仪器并交付给MEDVAMC使用； 第二架将在第四年初组装并交付给北大医院使用。这台仪器将会 设计用于快速获取荧光幅度图像，而不是断层扫描。当阶段 增强器和激光二极管的增益调制之间的差值保持不变，为稳态图像 对相位延迟的大小敏感的信号被捕获。如果我们引入零度和90度的相位 在调制激发光和探测器之间切换时，采集到两种不同的稳态图像。 这两个图像之间的差异导致 得到了无混叠的荧光振幅图像 环境直流光水平的影响。这种方法是 受雇于光照水平的透视下，与我们的工作 Mallinckrodt医学和洛夫莱斯研究所 20世纪90年代中期对犬的反应性淋巴结进行成像[90]。

Task Specific Project #2: FDPM Surgical Instrument for intraoperative guidance The goal of this Specific Task Project is to adapt our FDPM instrument for use in the surgical suite to locate and identify cancer positive LNs delineated by NIR dyes conjugated to targeting moieties as described in the primary project. In year three, the first instrument will be developed and delivered for use at the MEDVAMC; the second will be assembled and delivered for use at BTGH in the early part of year four. This instrument will be designed for rapidly acquired fluorescent amplitude images rather than tomography. When the phase difference between gain modulation of the intensifier and the laser diode is kept constant, a stead-state image is captured that is sensitive to the magnitude of the phase delay. If we introduce a zero and 90 degree phase change between the modulated excitation light and the detector, two different stead-state images are collected. The difference between these two images results in the fluorescent amplitude image is obtained without the confounding effect of the ambient DC light levels. This approach was employed under light levels of fluoroscopy with our work with Mallinckrodt Medical and the Lovelace Research Institute in the mid 1990s to image reactive lymph nodes in canines [90].