Functional Genomic Studies of Early Myogenic Differentiation
Functional Genomic Studies of Early Myogenic Differentiation
批准号:
7663827
负责人:
Joseph C Glorioso
金额:
$31.67万
依托单位国家:
美国
项目类别:
财政年份:
2008
资助国家:
美国
项目状态:
已结题
起止时间:
2008-06-01 至 2009-05-31
关键词:
A MouseAdultAliquotAppearanceBacteriaBacterial Artificial ChromosomesBiochemicalBiological AssayBiological ModelsCandidate Disease GeneCell Differentiation processCell LineCell LineageCell divisionCellsCloningCloning VectorsCodeCommitComplementComplementary DNAComplexConditionDNA BindingDNA LibraryDataDatabasesDefective VirusesDetectionDevelopmentES Cell LineEarly PromotersEatingElementsEmbryoEngineeringEnhancersEssential GenesEventExcisionExpression LibraryFigs - dietaryFluorescenceGene ExpressionGene TargetingGenesGenetic RecombinationGenomeGoalsGrowthGrowth FactorHSV vectorHerpesvirus 1ICP47IGF1 geneImageImmediate-Early GenesIn VitroInfectionInsulin-Like Growth Factor IJointsKineticsLIF geneLeftLibrariesLifeMethodsModelingMusMuscleMuscle CellsMuscle DevelopmentMuscle satellite cellNaturePathway interactionsPhenotypePlasmid Cloning VectorPlasmidsPlayPolymerase Chain ReactionPopulationProcessProductionProteinsRNARangeReporter GenesResearch PersonnelRoleSerumSignal TransductionSimplexvirusStagingStandards of Weights and MeasuresStem cellsSystemTK GeneTechnologyTestingTimeTissuesTrans-ActivatorsTranscriptional RegulationTransplantationVP 16Vero CellsVertebral columnViralViral ProteinsVirionVirusVirus ReplicationbasecDNA ExpressioncDNA Librarycell growthcellular engineeringcytotoxicdayembryonic stem cellengineering designexperiencefunctional genomicsinsightinterestleukemia inhibitory factormutantmyogenesisnectinnovelparticleprogenitorpromoterprotein expressionreceptorred fluorescent proteinresearch studystemtransgene expressionvector
中文摘要
该项目的总体目标是采用一种新的功能基因组学方法来识别基因
英文摘要
The overall goal of this project is to employ a novel functional genomics approach to identify genes
whose products induce the differentiation of mouse embryonic stem (ES) cells towards muscle cells. In
Aim 1, a highly defective, non-cytotoxic HSV mutant will be developed that is deficient for expression of
the immediate early (IE) genes and capable of replication in cells engineered to contain a
vector-inducible, trans-complementing ICP4 gene. In Aim 2, a panel of mouse ES cell lines will be created
in which either the EGFP or ICP4 gene will be recombined into the cellular genome juxtaposed to the
promoter that drives expression of one of the muscle differentiation factors Pax7, myf5, or myoD. Using
established culture conditions, the EGFP cell lines will be differentiated towards muscle cells and the
appearance of fluorescing cells will be correlated with detection of stage specific myocytic markers. In
Aim 3, the mutant virus will be engineered to contain a bacterial artificial chromosome and a
recombination system, "Gateway" (Invitrogen), which will then be used to introduce a mouse muscle
lprogenitor cell cDNA library into the vector. The vector library will be propagated in bacteria as single
lcopy plasmids, its complexity established by quantitative PCR for low and medium copy genes and
ltransfected into ICP4-complementing Vero cells for virus production. In Aim 4, pooled library vectors will
lbe propagated on complementing Vero cells in microtiter wells and replica plated onto the three myogenic
promoter-ICP4 ES cell lines. Progeny vector particles generated in cells expressing ICP4 by induction of
the resident myogenic promoter will be isolated by limiting dilution on complementing Vero cells and
rescreened on the panel of EGFP ES cell lines to determine whether the cellular factor is functional in
activating early and late stage myogenic factor promoters. The cDNA of activating vectors will be
sequenced, compared with known data bases for preliminary assessment of function and further
characterized for their ability to induce muscle cell markers using immunological and biochemical assays.
Ultimately, we hope to identify novel factors or signaling mechanisms that interact to control early muscle
cell development.
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Arming Oncolytic HSV Vectors to Induce Anti-GBM Immune Responses in Syngeneic Mice
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批准号:9927607
-
项目类别:
-
资助金额:$39.89万
-
财政年份:2018
-
负责人:Joseph C Glorioso
-
依托单位:
Arming Oncolytic HSV Vectors to Induce Anti-GBM Immune Responses in Syngeneic Mice
-
批准号:10409654
-
项目类别:
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资助金额:$38.92万
-
财政年份:2018
-
负责人:Joseph C Glorioso
-
依托单位:
Project 1: Arming Oncolytic HSV Vectors to Improve Virolysis in Syngeneic Mouse Models of GBM
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批准号:10019362
-
项目类别:
-
资助金额:$33.92万
-
财政年份:2013
-
负责人:Joseph C Glorioso
-
依托单位:
Project 1: Arming Oncolytic HSV Vectors to Improve Virolysis in Syngeneic Mouse Models of GBM
-
批准号:10491206
-
项目类别:
-
资助金额:$34.83万
-
财政年份:2013
-
负责人:Joseph C Glorioso
-
依托单位:
Project 1: Arming Oncolytic HSV Vectors to Improve Virolysis in Syngeneic Mouse Models of GBM
-
批准号:10251082
-
项目类别:
-
资助金额:$34.72万
-
财政年份:2013
-
负责人:Joseph C Glorioso
-
依托单位:
Project 1: Treatment of GBM using an oncolytic HSV engineered to improve immunogenic tumor destruction
-
批准号:10712280
-
项目类别:
-
资助金额:$34.32万
-
财政年份:2013
-
负责人:Joseph C Glorioso
-
依托单位:
Glycine Receptor Expression in Sensory Afferents to Modulate Pain Signaling
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批准号:8309978
-
项目类别:
-
资助金额:$36.05万
-
财政年份:2011
-
负责人:Joseph C Glorioso
-
依托单位:
Glycine Receptor Expression in Sensory Afferents to Modulate Pain Signaling
-
批准号:8186007
-
项目类别:
-
资助金额:$36.04万
-
财政年份:2011
-
负责人:Joseph C Glorioso
-
依托单位:
Glycine Receptor Expression in Sensory Afferents to Modulate Pain Signaling
-
批准号:8703184
-
项目类别:
-
资助金额:$35.71万
-
财政年份:2011
-
负责人:Joseph C Glorioso
-
依托单位:
Glycine Receptor Expression in Sensory Afferents to Modulate Pain Signaling
-
批准号:8520405
-
项目类别:
-
资助金额:$34.8万
-
财政年份:2011
-
负责人:Joseph C Glorioso
-
依托单位:
Functional Genomic Studies of Early Myogenic Differentiation
-
批准号:7509215
-
项目类别:
-
资助金额:$7.17万
-
财政年份:2007
-
负责人:Joseph C Glorioso
-
依托单位:
Glioma Therapy Using Targeted Oncolytic HSV Vectors
-
批准号:7019603
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项目类别:
-
资助金额:$34.68万
-
财政年份:2006
-
负责人:Joseph C Glorioso
-
依托单位:
NOVEL MODULATORS OF THE VANILLOID RECEPTOR
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批准号:7083038
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项目类别:
-
资助金额:$41.34万
-
财政年份:2006
-
负责人:Joseph C Glorioso
-
依托单位:
Glioma Therapy Using Targeted Oncolytic HSV Vectors
-
批准号:7579909
-
项目类别:
-
资助金额:$35.92万
-
财政年份:2006
-
负责人:Joseph C Glorioso
-
依托单位:
ADMINISTRATIVE
-
批准号:7083034
-
项目类别:
-
资助金额:$5.13万
-
财政年份:2006
-
负责人:Joseph C Glorioso
-
依托单位:
Glioma Therapy Using Targeted Oncolytic HSV Vectors
-
批准号:7386790
-
项目类别:
-
资助金额:$34.94万
-
财政年份:2006
-
负责人:Joseph C Glorioso
-
依托单位:
Glioma Therapy Using Targeted Oncolytic HSV Vectors
-
批准号:7225552
-
项目类别:
-
资助金额:$34.66万
-
财政年份:2006
-
负责人:Joseph C Glorioso
-
依托单位:
Glioma Therapy Using Targeted Oncolytic HSV Vectors
-
批准号:7774399
-
项目类别:
-
资助金额:$36.57万
-
财政年份:2006
-
负责人:Joseph C Glorioso
-
依托单位:
Administrative Core
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批准号:7144439
-
项目类别:
-
资助金额:$7.65万
-
财政年份:2005
-
负责人:Joseph C Glorioso
-
依托单位:
Heme Oxygenas Gene Therapy of Heart Ischemia-reperfusion
-
批准号:7139392
-
项目类别:
-
资助金额:$36.97万
-
财政年份:2005
-
负责人:Joseph C Glorioso
-
依托单位:
海外基金